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PMID: 8642317 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of apoptosis-resistant antigen-specific T cells in vivo.

The Journal of experimental medicine ·Vol. 183 ·No. 5 ·1996-05-01 ·Pages 2065-73

Zhang L, Miller RG, Zhang J

Abstract

Clonal deletion via activation-induced apoptosis (AIA) of antigen-specific T cells (ASTC) plays a very important role in the induction of peripheral tolerance. However, none of the studies performed so far has shown a complete deletion of ASTC, a small population always persisting in the periphery. The mechanism by which this small population of ASTC escapes AIA has not been determined. Since the existence of these ASTC may influence the outcome of autoimmune diseases and long-term graft survival, we have characterized the properties of these residual ASTC in vivo with the objective of determining mechanisms that may contribute to their persistence. It was found that the resistance of the residual ASTC to AIA is not due to lack of activation or Fas/Fas-L expression. Compared to those susceptible to AIA, the residual ASTC express a high level of Th2-type cytokines that may help them to escape from AIA. Furthermore, they are able to suppress proliferation of other ASTC, suggesting they may, in fact, prolong tolerance in vivo.

MeSH Terms
Animals Apoptosis Base Sequence Cytotoxicity, Immunologic DNA Primers Flow Cytometry Immune Tolerance Immunotherapy, Adoptive Lymph Nodes/immunology Lymphocyte Activation Lymphocyte Depletion Major Histocompatibility Complex Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/analysis Spleen/immunology T-Lymphocyte Subsets/immunology T-Lymphocytes/immunology fas Receptor/biosynthesis
Chemicals
DNA Primers RNA, Messenger fas Receptor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhang L
Department of Cellular and Molecular Pathology, Toronto Hospital Research Institute, University of Toronto, Canada.
Miller R G
Zhang J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.183.5.2065
Published
1996-05-01
Pages
2065-73
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192552
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.183.5.2065 PMC Full Text View journal details
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