Home LiteratureArticle Details
PMID: 8645229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Translocation of annexin I to plasma membranes and phagosomes in human neutrophils upon stimulation with opsonized zymosan: possible role in phagosome function.

The Biochemical journal ·Vol. 316 ( Pt 1) ·1996-05-15 ·Pages 35-42

Kaufman M, Leto T, Levy R

Abstract

Annexin I in the cytosol of resting neutrophils was translocated to the plasma membranes upon addition of opsonized zymosan (OZ). Maximum translocation could be detected 1 min after stimulation with OZ, and decreased thereafter. Subcellular fractionation studies demonstrated that annexin I could not be detected in the granule fractions in either resting or activated cells, but was found in association with the phagosome fraction. The marked translocation of annexin I was unique to OZ, since formyl-Met-Leu-Phe induced only slight translocation of annexin I to the plasma membranes, and phorbol 12-myristate 13-acetate had no effect at all. The mechanism regulating the translocation of annexin I is not clear. Annexin I is not phosphorylated in resting or stimulated cells. The correlation between the elevation in the intracellular calcium ion concentration ([Ca2+]i) and the degree of translocation of annexin I to the plasma membranes induced by the different stimuli, together with the inhibition of these processes by the addition of EGTA, indicate that the translocation of annexin I can probably be attributed to the rise in [Ca2+]i. However, this cannot be the sole mechanism since ionomycin, which caused an increase in [CA2+]i similar to that induced by OZ, was less efficient than OZ in inducing translocation of annexin I. The induction of annexin I translocation to the plasma membrane by OZ, which was the only agent that induced phagosome formation, and the detection of annexin I in the phagosome fraction, suggest that annexin I participates in phagosome function.

MeSH Terms
Annexin A1/blood Cell Fractionation Cell Membrane/metabolism Cytochrome b Group/blood Humans In Vitro Techniques Kinetics NADPH Oxidases Neutrophils/drug effects,physiology Peroxidase/blood Phagosomes/metabolism Sodium-Potassium-Exchanging ATPase/blood Superoxides/blood Tetradecanoylphorbol Acetate/pharmacology Time Factors Zymosan/pharmacology
Chemicals
Annexin A1 Cytochrome b Group Superoxides Zymosan cytochrome b558 Peroxidase NADPH Oxidases Sodium-Potassium-Exchanging ATPase Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kaufman M
Laboratory of Infectious Diseases, Faculty of Health Sciences, Soroka Medical Center of Kupat Holim, Ben-Gurion University of the Negev, Beer Sheva, Israel.
Leto T
Levy R
References (43)
43 references, click to expand
  1. Intracellular control of human neutrophil secretion. I. C5a-induced stimulus-specific desensitization and the effects of cytochalasin B.
    J Immunol. 1978 Sep;121(3):851-5 PMID: 211165
  2. Production of myeloid cell cytosols functionally and immunochemically deficient in the 47 kDa or 67 kDa NADPH oxidase cytosolic factors.
    Biochem Biophys Res Commun. 1990 Aug 16;170(3):1114-20 PMID: 2167670
  3. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation.
    J Cell Biol. 1983 Jul;97(1):52-61 PMID: 6408102
  4. Isolation of a calcium-dependent 35-kilodalton substrate for the epidermal growth factor receptor/kinase from A-431 cells.
    J Biol Chem. 1984 Feb 25;259(4):2636-45 PMID: 6698984
  5. Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity.
    Nature. 1986 Mar 6-12;320(6057):77-81 PMID: 2936963
  6. Two human 35 kd inhibitors of phospholipase A2 are related to substrates of pp60v-src and of the epidermal growth factor receptor/kinase.
    Cell. 1986 Jul 18;46(2):191-9 PMID: 3013422
  7. Inhibition of phospholipase A2 by "lipocortins" and calpactins. An effect of binding to substrate phospholipids.
    J Biol Chem. 1987 Feb 5;262(4):1698-705 PMID: 2948959
  8. Characterization of lipocortin I and an immunologically unrelated 33-kDa protein as epidermal growth factor receptor/kinase substrates and phospholipase A2 inhibitors.
    J Biol Chem. 1987 May 15;262(14):6921-30 PMID: 3032981
  9. Synexin-like proteins from human polymorphonuclear leukocytes. Identification and characterization of granule-aggregating and membrane-fusing activities.
    J Biol Chem. 1987 Jun 5;262(16):7850-8 PMID: 2953727
  10. Purification and characterization of a 32-kDa phospholipase A2 inhibitory protein (lipocortin) from human peripheral blood mononuclear cells.
    FEBS Lett. 1987 Jul 13;219(1):169-75 PMID: 2954855
  11. The effect of inhibition of both diacylglycerol metabolism and phospholipase A2 activity on superoxide generation by human neutrophils.
    FEBS Lett. 1988 Jul 4;234(1):235-40 PMID: 2839364
  12. Phospholipase A2 activation in human neutrophils. Differential actions of diacylglycerols and alkylacylglycerols in priming cells for stimulation by N-formyl-Met-Leu-Phe.
    J Biol Chem. 1988 Nov 15;263(32):16787-95 PMID: 2846550
  13. Differentiation of HL-60 cells is associated with an increase in the 35-kDa protein lipocortin I.
    J Cell Physiol. 1988 Dec;137(3):402-10 PMID: 2973467
  14. Ca2+-dependent phospholipid- (and membrane-) binding proteins.
    Biochemistry. 1988 Sep 6;27(18):6645-53 PMID: 2973805
  15. Induction of the respiratory burst in HL-60 cells. Correlation of function and protein expression.
    J Immunol. 1990 Oct 15;145(8):2595-601 PMID: 2170520
  16. Differential subcellular distribution of p36 (the heavy chain of calpactin I) and other annexins in the adrenal medulla.
    J Neurochem. 1991 Feb;56(2):469-78 PMID: 1824861
  17. Annexin III translocates to the periphagosomal region when neutrophils ingest opsonized yeast.
    J Immunol. 1991 May 1;146(9):3110-4 PMID: 1826705
  18. Ca(2+)-dependent translocation of cytosolic proteins to isolated granule subpopulations and plasma membrane from human neutrophils.
    FEBS Lett. 1992 Jun 15;304(2-3):195-7 PMID: 1319926
  19. Annexin I-mediated vesicular aggregation: mechanism and role in human neutrophils.
    Biochemistry. 1992 Jul 21;31(28):6372-82 PMID: 1385975
  20. Human neutrophil annexin I promotes granule aggregation and modulates Ca(2+)-dependent membrane fusion.
    J Clin Invest. 1992 Aug;90(2):537-44 PMID: 1386611
  21. Regulation of the chromaffin granule aggregating activity of annexin I by phosphorylation.
    Biochemistry. 1992 Oct 20;31(41):9934-9 PMID: 1390776
  22. The annexins and exocytosis.
    Science. 1992 Nov 6;258(5084):924-31 PMID: 1439804
  23. Modulation of IL-1-induced neutrophil migration by dexamethasone and lipocortin 1.
    J Immunol. 1993 Feb 1;150(3):992-9 PMID: 8423349
  24. New insights on mammalian phospholipase A2(s); comparison of arachidonoyl-selective and -nonselective enzymes.
    FASEB J. 1993 Feb 1;7(2):339-48 PMID: 8440410
  25. Annexin I interactions with human neutrophil specific granules: fusogenicity and coaggregation with plasma membrane vesicles.
    Biochim Biophys Acta. 1993 Apr 22;1147(2):177-84 PMID: 8476911
  26. Annexin 5 as a potential regulator of annexin 1 phosphorylation by protein kinase C. In vitro inhibition compared with quantitative data on annexin distribution in human endothelial cells.
    Biochem J. 1993 Jun 15;292 ( Pt 3):759-65 PMID: 8318006
  27. Regulation of annexin I-dependent aggregation of phospholipid vesicles by protein kinase C.
    Biochem J. 1993 Sep 15;294 ( Pt 3):801-7 PMID: 8379935
  28. The requirement for phospholipase A2 for activation of the assembled NADPH oxidase in human neutrophils.
    Biochem J. 1994 Jan 1;297 ( Pt 1):217-23 PMID: 8280102
  29. The effects of PMA and TFP and alterations in intracellular pH and calcium concentration on the membrane associations of phospholipid-binding proteins fodrin, protein kinase C and annexin II in cultured MDCK cells.
    Biochim Biophys Acta. 1994 Jan 3;1189(1):21-30 PMID: 8305455
  30. The requirement of p47 phosphorylation for activation of NADPH oxidase by opsonized zymosan in human neutrophils.
    Biochim Biophys Acta. 1994 Feb 17;1220(3):253-60 PMID: 8305497
  31. Calcium-induced translocation of annexins to subcellular organelles of human neutrophils.
    Biochem J. 1994 Jun 1;300 ( Pt 2):325-30 PMID: 8002935
  32. Expression of annexin I in freshly isolated human epidermal cells and in cultured keratinocytes.
    Arch Dermatol Res. 1994;286(5):268-72 PMID: 8060156
  33. Association of Rap1a and Rap1b proteins with late endocytic/phagocytic compartments and Rap2a with the Golgi complex.
    J Cell Sci. 1994 Jun;107 ( Pt 6):1661-70 PMID: 7962206
  34. Annexin 3 is associated with cytoplasmic granules in neutrophils and monocytes and translocates to the plasma membrane in activated cells.
    Biochem J. 1994 Oct 15;303 ( Pt 2):481-7 PMID: 7526843
  35. Arachidonic acid increases the activity of the assembled NADPH oxidase in cytoplasmic membranes and endosomes.
    Biochim Biophys Acta. 1993 Mar 10;1176(1-2):51-8 PMID: 7680903
  36. A role for protein kinase C-mediated phosphorylation in the mobilization of arachidonic acid in mouse macrophages.
    Biochim Biophys Acta. 1989 Jan 17;1010(1):78-87 PMID: 2491791
  37. Inhibition of O2- generation by dexamethasone is mimicked by lipocortin I in alveolar macrophages.
    J Clin Invest. 1989 Jun;83(6):1936-40 PMID: 2542378
  38. The annexin family of calcium-binding proteins. Review article.
    Cell Calcium. 1989 Jan;10(1):1-10 PMID: 2659190
  39. Superoxide generation is inhibited by phospholipase A2 inhibitors. Role for phospholipase A2 in the activation of the NADPH oxidase.
    Biochem J. 1989 Nov 15;264(1):249-55 PMID: 2557829
  40. Characterization of Ca2(+)-dependent phospholipid binding, vesicle aggregation and membrane fusion by annexins.
    Biochem J. 1990 Feb 15;266(1):195-200 PMID: 2138016
  41. Stimulation of the respiratory burst in peripheral blood monocytes by lipoteichoic acid. The involvement of calcium ions and phospholipase A2.
    J Immunol. 1990 Apr 1;144(7):2665-70 PMID: 2156929
  42. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src.
    Science. 1990 May 11;248(4956):727-30 PMID: 1692159
  43. Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader.
    J Immunol Methods. 1981;46(2):211-26 PMID: 6273471
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3160035
Published
1996-05-15
Pages
35-42
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1217346
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3160035 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2