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PMID: 8649372 Published · ppublish English Journal Article

The Saccharomyces cerevisiae Start-specific transcription factor Swi4 interacts through the ankyrin repeats with the mitotic Clb2/Cdc28 kinase and through its conserved carboxy terminus with Swi6.

Molecular and cellular biology ·Vol. 16 ·No. 6 ·1996-06-00 ·Pages 2647-55

Siegmund RF, Nasmyth KA

Abstract

At a point in late G1 termed Start, yeast cells enter S phase, duplicate their spindle pole bodies, and form buds. These events require activation of Cdc28 kinase by G1 cyclins. Swi4 associates with Swi6 to form the SCB-binding factor complex which activates G1 cyclin genes CLN1 and CLN2 in late G1. In G2 and M phases, the transcriptional activity of SCB-binding factor is repressed by the mitotic Clb2/Cdc28 kinase. Mbp1, a transcription factor related to Swi4, forms the MCB-binding factor complex with Swi6, which activates DNA synthesis genes and S-phase cyclin genes CLB5 and CLB6 in late G1. Clb2/Cdc28 kinase is not required for the repression of MCB-binding factor transcriptional activity in G2 and M phase. We show here that the Swi4 carboxy terminus is sufficient for interaction with Swi6 in vitro. A carboxy-terminal domain of Swi6 is required and sufficient for interaction with Swi4. The carboxy terminus of Mbp1 is sufficient for interaction with Swi6, and the carboxy terminus of Swi6 is required for interaction with Mbp1. By coimmunoprecipitation, we show that Swi4 but not Mbp1 interacts with Clb2/Cdc28 kinase in vivo during the G2 and M phases of the cell cycle. We demonstrate that the ankyrin repeats of Swi4 mediate the interaction with Clb2/Cdc28 kinase. The ankyrin repeats constitute a domain by which a cell cycle-specific transcription factor can interact with cyclin-dependent kinase complexes, thus enabling it to link its transcriptional activity to cell cycle progression.

MeSH Terms
Amino Acid Sequence Ankyrins/genetics CDC28 Protein Kinase, S cerevisiae/genetics,metabolism Cell Cycle Conserved Sequence Cyclin B Cyclins/metabolism DNA-Binding Proteins Fungal Proteins/genetics,metabolism Mitosis Models, Biological Molecular Sequence Data Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Transcription Factors/genetics,metabolism
Chemicals
Ankyrins CLB2 protein, S cerevisiae Cyclin B Cyclins DNA-Binding Proteins Fungal Proteins SWI4 protein, S cerevisiae SWI6 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors CDC28 Protein Kinase, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Siegmund R F
Research Institute of Molecular Pathology, Vienna, Austria.
Nasmyth K A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.6.2647
Published
1996-06-00
Pages
2647-55
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231255
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.6.2647 PMC Full Text View journal details
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