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PMID: 8660378 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence, chromosomal assignment and mRNA expression of mouse hypoxia-inducible factor-1 alpha.

Biochemical and biophysical research communications ·Vol. 223 ·No. 1 ·1996-06-05 ·Pages 54-9

Wenger RH, Rolfs A, Marti HH, Guénet JL, Gassmann M

Abstract

The heterodimeric hypoxia-inducible transcription factor HIF-1 is involved in the oxygen-regulated transcription of several genes including erythropoietin. Cloning and sequencing of the alpha-subunit of mouse HIF-1 cDNA revealed a 90% overall homology to human HIF-l alpha but lack of any similarity in the 5' untranslated region and translational start site. Mouse HIF-1 alpha is encoded by an evolutionary conserved single-copy gene located on chromosome 12. We found a widespread constitutive expression of mouse HIf-1 alpha mRNA which was particularly high in lung and kidney. Despite a strong erythropoietin induction, HIF-1 alpha mRNA concentrations were not upregulated in hypoxic mouse tissues.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Biological Evolution Cell Line Chromosome Mapping Conserved Sequence DNA Primers DNA, Complementary DNA-Binding Proteins/biosynthesis,chemistry HeLa Cells Humans Hypoxia Hypoxia-Inducible Factor 1 Hypoxia-Inducible Factor 1, alpha Subunit Kidney/metabolism Liver Neoplasms, Experimental Lung/metabolism Macromolecular Substances Mice Molecular Sequence Data Nuclear Proteins/biosynthesis,chemistry Polymerase Chain Reaction RNA, Messenger/biosynthesis Sequence Homology, Nucleic Acid Transcription Factors/biosynthesis Transcription, Genetic Tumor Cells, Cultured

Chemicals

DNA Primers DNA, Complementary DNA-Binding Proteins HIF1A protein, human Hif1a protein, mouse Hypoxia-Inducible Factor 1 Hypoxia-Inducible Factor 1, alpha Subunit Macromolecular Substances Nuclear Proteins RNA, Messenger Transcription Factors

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Wenger R H

Physiologisches Institut, Universität Zürich-Irchel, Switzerland.

Rolfs A

Marti H H

Guénet J L

Gassmann M

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.1996.0845

Published

1996-06-05

Pages

54-9

Language

English

Region

United States

NLM ID

0372516

Subset

Databases

GENBANK

X95580

External Links

PubMed source DOI: 10.1006/bbrc.1996.0845 View journal details

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Nucleotide sequence, chromosomal assignment and mRNA expression of mouse hypoxia-inducible factor-1 alpha.

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