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PMID: 866192 Published · ppublish English Journal Article

Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger.

Nucleic acids research ·Vol. 4 ·No. 4 ·1977-04-00 ·Pages 801-11

Destrée OH, Haenni AL, Birnstiel ML

Abstract

RNA extracted from ovaries of adult Xenopus laevis and introduced into a wheat germ cell-free system, directs the synthesis of polypeptides co-electrophoresing with all five histones. By frationation of total ovary RNA through Sephadex G-200 and polyacrylamide gel, RNA was isolated that is translated into histones 4 (H4) only. In addition, this RNA labelled in vitro with [I125] hybridizes to a restriction fragment of cloned sea urchin DNA known to contain the H4 gene.

MeSH Terms
Animals Cell-Free System Clone Cells DNA Female Genes Histones/biosynthesis Nucleic Acid Hybridization Ovary/analysis Protein Biosynthesis RNA/isolation & purification RNA, Messenger/isolation & purification,metabolism Sea Urchins Triticum/metabolism Xenopus
Chemicals
Histones RNA, Messenger RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Destrée O H
Haenni A L
Birnstiel M L
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.4.801
Published
1977-04-00
Pages
801-11
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342485
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.4.801 PMC Full Text View journal details
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