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PMID: 8669721 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An E-selectin-IgG chimera supports sialylated moiety dependent adhesion of colon carcinoma cells under fluid flow.

Annals of biomedical engineering ·Vol. 24 ·No. 1 ·1996-00-00 ·Pages 87-98

Goetz DJ, Brandley BK, Hammer DA

Abstract

To further characterize the molecular mechanisms that govern carcinoma cell adhesion to stimulated endothelium, we studied the adhesion of a human colon carcinoma cell line, KM12-L4, to an E-selectin-IgG1 chimera and interleukin (IL)-1 beta-stimulated human umbilical vein endothelial cells (HUVEC) under in vitro fluid flow conditions. Between 0.6 and 1.8 dynes/cm2, KM12-L4 cells attach to and roll on IL-1 beta-stimulated HUVEC. The adhesion is E-selectin dependent and diminished upon pretreatment of the KM12-L4 cells with neuraminidase (neuraminidase sensitive). Between 0.7 and 1.8 dynes/cm2, surfaces coated with an E-selectin-IgG1 chimera support attachment and rolling of KM12-L4 cells. The adhesion to the E-selectin-IgG1 chimera is blocked by an antibody to the lectin domain of E-selectin and is neuraminidase sensitive. Rolling KM12-L4 cells exhibit variable velocity motion over both IL-1 beta-stimulated HUVEC and E-selectin-IgG1 chimera-coated surfaces. Our results provide the first direct evidence that sialylated moieties are involved in the adhesion of carcinoma cells to IL-1 beta-stimulated endothelium under flow conditions; E-selectin-IgG1 chimeras can support cell attachment and rolling under defined flow conditions; the topology of the endothelium is not the sole cause of variable velocity motion observed in cell rolling systems.

MeSH Terms
Cell Adhesion/drug effects,physiology Colonic Neoplasms/physiopathology E-Selectin/physiology Endothelium, Vascular/physiology Flow Cytometry Humans Immunoglobulin G/pharmacology,physiology Interleukin-1/pharmacology,physiology Neuraminidase/metabolism,pharmacology Recombinant Fusion Proteins/metabolism Rheology Surface Properties Tumor Cells, Cultured/physiology Umbilical Veins/physiology
Chemicals
E-Selectin Immunoglobulin G Interleukin-1 Recombinant Fusion Proteins Neuraminidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goetz D J
School of Chemical Engineering, Cornell University, Ithaca, NY 14853, USA.
Brandley B K
Hammer D A
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Article Info
Journal
Annals of biomedical engineering
Abbr.
Ann Biomed Eng
ISSN
0090-6964
DOI
10.1007/BF02770998
Published
1996-00-00
Pages
87-98
Language
English
Region
United States
NLM ID
0361512
Subset
IM
Grants
NHLBI NIH HHS · HL 18208 · United States
NIGMS NIH HHS · T23-GM08384 · United States
External Links
PubMed source DOI: 10.1007/BF02770998 View journal details
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