Home LiteratureArticle Details
PMID: 8670116 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning, sequencing and expression of the transferrin-binding protein 1 gene from Actinobacillus pleuropneumoniae.

The Biochemical journal ·Vol. 315 ( Pt 1) ·1996-04-01 ·Pages 257-64

Daban M, Medrano A, Querol E

Abstract

Two outer-membrane proteins are involved in the uptake of iron from transferrin by certain Gram-negative bacteria, transferrin-binding proteins 1 and 2. The gene encoding transferrin-binding protein 1 from a serotype 1 isolate of the Gram-negative pathogen Actinobacillus pleuropneumoniae was cloned, and a fragment encoding 700 amino acids of Tbp1 was expressed in Escherichia coli. We also report here sequencing of the tbpl gene and a comparison of the deduced amino acid sequence with Tbpls from related species. The predicted polypeptide product of tbpl is a 106 kDa protein with a 22-residue signal peptide.

MeSH Terms
Actinobacillus pleuropneumoniae/genetics,metabolism Amino Acid Sequence Bacterial Proteins/biosynthesis,genetics,isolation & purification Base Sequence Carrier Proteins/biosynthesis,genetics,isolation & purification Cloning, Molecular Escherichia coli/genetics,metabolism Gene Expression Genes, Bacterial Iron-Binding Proteins Molecular Sequence Data Sequence Homology, Amino Acid Transferrin-Binding Proteins
Chemicals
Bacterial Proteins Carrier Proteins Iron-Binding Proteins Transferrin-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Daban M
Institut de Biologia Fonamental and Department de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Barcelona, Spain.
Medrano A
Querol E
References (40)
40 references, click to expand
  1. Iron assimilation and storage in prokaryotes.
    J Gen Microbiol. 1992 Dec;138(12):2475-83 PMID: 1487719
  2. Correlation between the ability of Haemophilus paragallinarum to acquire ovotransferrin-bound iron and the expression of ovotransferrin-specific receptors.
    Avian Dis. 1992 Jul-Sep;36(3):655-63 PMID: 1417595
  3. Characterization of the transferrin-iron uptake system in Neisseria meningitidis.
    FEMS Microbiol Lett. 1993 Sep 1;112(2):159-65 PMID: 8405958
  4. Porins and specific diffusion channels in bacterial outer membranes.
    J Biol Chem. 1994 Feb 11;269(6):3905-8 PMID: 7508433
  5. Fur regulon in gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay.
    J Mol Biol. 1994 Feb 18;236(2):531-45 PMID: 8107138
  6. In vivo human immune response to transferrin-binding protein 2 and other iron-regulated proteins of Neisseria meningitidis.
    FEMS Immunol Med Microbiol. 1994 Jan;8(1):63-8 PMID: 8156052
  7. Cloning and sequence analysis of the fur gene encoding an iron-regulatory protein of Neisseria meningitidis.
    Gene. 1994 Apr 8;141(1):139-40 PMID: 8163167
  8. A functional tonB gene is required for both utilization of heme and virulence expression by Haemophilus influenzae type b.
    Infect Immun. 1994 Jun;62(6):2470-7 PMID: 8188372
  9. Immune responses in humans and animals to meningococcal transferrin-binding proteins: implications for vaccine design.
    Infect Immun. 1994 Jul;62(7):2984-90 PMID: 8005685
  10. The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan.
    Mol Microbiol. 1994 Apr;12(2):333-4 PMID: 8057857
  11. Transferrin receptors on ruminant pathogens vary in their interaction with the C-lobe and N-lobe of ruminant transferrins.
    Can J Microbiol. 1994 Jul;40(7):532-40 PMID: 8076248
  12. Immune response of Brazilian children to a Neisseria meningitidis serogroup B outer membrane protein vaccine: comparison with efficacy.
    Infect Immun. 1994 Oct;62(10):4419-24 PMID: 7927704
  13. Observation of binding and polymerization of Fur repressor onto operator-containing DNA with electron and atomic force microscopes.
    Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11816-20 PMID: 7991541
  14. Signal peptides: exquisitely designed transport promoters.
    Mol Microbiol. 1994 Sep;13(5):765-73 PMID: 7815936
  15. Evaluation of transferrin-binding protein 2 within the transferrin-binding protein complex as a potential antigen for future meningococcal vaccines.
    Infect Immun. 1995 Mar;63(3):884-90 PMID: 7868259
  16. Identification and characterization of genes encoding the human transferrin-binding proteins from Haemophilus influenzae.
    Infect Immun. 1995 Apr;63(4):1201-10 PMID: 7890373
  17. Comparison of the class-1 outer membrane protein from B:15:P1.16 Neisseria meningitidis strains isolated from patients previously immunized with a serogroup B outer membrane protein vaccine in Norway.
    Microb Pathog. 1994 Dec;17(6):425-30 PMID: 7752883
  18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  19. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  20. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  21. Construction and expression of hybrid plasmids containing the Escherichia coli glyA genes.
    Gene. 1981 Jun-Jul;14(1-2):63-72 PMID: 7021324
  22. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  23. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
    J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
  24. Effect of iron restriction on the outer membrane proteins of Actinobacillus (Haemophilus) pleuropneumoniae.
    Infect Immun. 1989 Mar;57(3):798-804 PMID: 2917787
  25. Fast and sensitive multiple sequence alignments on a microcomputer.
    Comput Appl Biosci. 1989 Apr;5(2):151-3 PMID: 2720464
  26. Identification of the transferrin- and lactoferrin-binding proteins in Haemophilus influenzae.
    J Med Microbiol. 1989 Jun;29(2):121-30 PMID: 2543820
  27. Utilization of transferrin-bound iron by Haemophilus species of human and porcine origins.
    FEMS Microbiol Lett. 1989 Nov;53(1-2):123-7 PMID: 2533128
  28. Receptors for transferrin in pathogenic bacteria are specific for the host's protein.
    Can J Microbiol. 1990 Feb;36(2):145-7 PMID: 2110858
  29. Iron acquisition in Pasteurella haemolytica: expression and identification of a bovine-specific transferrin receptor.
    Infect Immun. 1990 Jul;58(7):2091-7 PMID: 2365453
  30. Identification and characterization of a porcine-specific transferrin receptor in Actinobacillus pleuropneumoniae.
    Mol Microbiol. 1990 Jul;4(7):1173-9 PMID: 2233254
  31. Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein.
    J Mol Biol. 1991 Mar 5;218(1):141-8 PMID: 1848301
  32. Response of Haemophilus somnus to iron limitation: expression and identification of a bovine-specific transferrin receptor.
    Microb Pathog. 1990 Dec;9(6):397-406 PMID: 2151461
  33. Cloning and expression of a transferrin-binding protein from Actinobacillus pleuropneumoniae.
    Infect Immun. 1992 Mar;60(3):892-8 PMID: 1541562
  34. Porins and specific channels of bacterial outer membranes.
    Mol Microbiol. 1992 Feb;6(4):435-42 PMID: 1373213
  35. Iron acquisition in Haemophilus influenzae: receptors for human transferrin.
    J Infect Dis. 1992 Jun;165 Suppl 1:S103-4 PMID: 1588135
  36. Characterization of two genes encoding distinct transferrin-binding proteins in different Actinobacillus pleuropneumoniae isolates.
    Infect Immun. 1992 Aug;60(8):3253-61 PMID: 1639494
  37. Crystal structures explain functional properties of two E. coli porins.
    Nature. 1992 Aug 27;358(6389):727-33 PMID: 1380671
  38. Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to TonB-dependent outer membrane receptors.
    J Bacteriol. 1992 Sep;174(18):5788-97 PMID: 1325963
  39. Formation of a gated channel by a ligand-specific transport protein in the bacterial outer membrane.
    Science. 1992 Oct 16;258(5081):471-5 PMID: 1411544
  40. Cloning and characterization of Neisseria meningitidis genes encoding the transferrin-binding proteins Tbp1 and Tbp2.
    Gene. 1993 Aug 16;130(1):73-80 PMID: 8344530
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3150257
Published
1996-04-01
Pages
257-64
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1217180
Subset
IM
Databases
GENBANK
Z49708
External Links
PubMed source DOI: 10.1042/bj3150257 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2