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PMID: 8670164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein kinase C isoforms epsilon, eta, delta and zeta in murine adipocytes: expression, subcellular localization and tissue-specific regulation in insulin-resistant states.

The Biochemical journal ·Vol. 316 ( Pt 3) ·1996-06-15 ·Pages 865-71

Frevert EU, Kahn BB

Abstract

The Ca(2+)-insensitive protein kinase C (PKC) isoforms epsilon, eta, delta and zeta are possible direct downstream targets of phosphatidylinositol 3-kinase (P13-K), and might therefore be involved in insulin signalling. Although isoform-specific changes in PKC expression have been reported for skeletal muscle and liver in insulin-resistant states, little is known about these isoforms in adipocytes. Therefore we studied (1) expression and subcellular localization of these isoforms in murine adipocytes, (2) translocation of specific isoforms to membranes in response to treatment with insulin and phorbol 12-myristate 13-acetate (PMA) and (3) regulation of expression in insulin-resistant states. The PKC isoforms epsilon, eta, delta and zeta are expressed in adipocytes. Immunoreactivity for all isoforms is higher in the membranes than in the cytosol, but subcellular fractionation by differential centrifugation shows an isoform-specific distribution within the membrane fractions. PMA treatment of adipocytes induces translocation of PKC-epsilon and -delta from the cytosol to the membrane fractions. Insulin treatment does not alter the subcellular distribution of any of the isoforms. 3T3-L1 adipocytes express PKC-epsilon and -zeta, and PKC-epsilon expression increases with differentiation from preadipocytes to adipocytes. PKC-epsilon expression decreases in an adipose-specific and age/obesity-dependent manner in two insulin-resistant models, the brown-adipose-tissue-deficient mouse and db/db mouse compared with control mice. We conclude that, although none of the isoforms investigated seems to be activated by insulin, the decrease in PKC-epsilon expression might contribute to metabolic alterations in adipocytes associated with insulin resistance and obesity.

MeSH Terms
3T3 Cells Adipocytes/cytology,enzymology Adipose Tissue/enzymology Animals Cell Differentiation Cell Fractionation Cell Membrane/enzymology Cells, Cultured Cytosol/enzymology Diet Gene Expression Regulation, Enzymologic Insulin Resistance Isoenzymes/analysis,biosynthesis Mice Mice, Inbred Strains Obesity/enzymology Protein Kinase C/analysis,biosynthesis Protein Kinase C-delta Protein Kinase C-epsilon Rats Rats, Sprague-Dawley Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Isoenzymes Prkcd protein, mouse Prkcd protein, rat Prkce protein, mouse Prkce protein, rat protein kinase C eta protein kinase C zeta Protein Kinase C Protein Kinase C-delta Protein Kinase C-epsilon Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Frevert E U
Department of Medicine, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215, USA.
Kahn B B
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1996-06-15
Pages
865-71
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1217430
Subset
IM
Grants
NIDDK NIH HHS · R01 DK043051 · United States
NIDDK NIH HHS · P30DK46200 · United States
NIDDK NIH HHS · R01DK43051 · United States
NIDDK NIH HHS · R01DK46930 · United States
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