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PMID: 8670883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ca2+ triggers massive exocytosis in Chinese hamster ovary cells.

The EMBO journal ·Vol. 15 ·No. 15 ·1996-08-01 ·Pages 3787-91

Coorssen JR, Schmitt H, Almers W

Abstract

We have tracked the cell surface area of CHO cells by measuring the membrane capacitance, Cm. An increase in cytosolic [Ca2+], [Ca2+]i, increased the cell surface area by 20-30%. At micromolar [Ca2+]i the increase occurred in minutes, while at 20 microM or higher [Ca2+]i it occurred in seconds and was transient. GTPgammaS caused a 3% increase even at 0.1 microM [Ca2+]i. We conclude that CHO cells, previously thought capable only of constitutive exocytosis, can perform Ca2+-triggered exocytosis that is both massive and rapid. Ca2+-triggered exocytosis was also observed in 3T3 fibroblasts. Our findings add evidence to the view that Ca induces exocytosis in cells other than known secretory cells.

MeSH Terms
Animals CHO Cells Calcium/physiology Cricetinae Cricetulus Exocytosis/drug effects Female Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Magnesium/pharmacology Ovary/cytology,drug effects,metabolism Photolysis
Chemicals
Guanosine 5'-O-(3-Thiotriphosphate) Magnesium Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Coorssen J R
Abteilung Molekulare Zellforschung, Max-Planck-Institut fur Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany.
Schmitt H
Almers W
References (31)
31 references, click to expand
  1. Exocytosis of pinocytic contents by Chinese hamster ovary cells.
    J Cell Biol. 1982 Jun;93(3):632-7 PMID: 7118995
  2. Calcium-regulated exocytosis is required for cell membrane resealing.
    J Cell Biol. 1995 Dec;131(6 Pt 2):1747-58 PMID: 8557742
  3. Capacitance measurements reveal stepwise fusion events in degranulating mast cells.
    Nature. 1984 Nov 29-Dec 5;312(5993):453-5 PMID: 6504157
  4. Regulation of transepithelial H+ transport by exocytosis and endocytosis.
    Annu Rev Physiol. 1986;48:153-61 PMID: 2423020
  5. Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi.
    Annu Rev Biochem. 1987;56:829-52 PMID: 3304148
  6. Gradual and stepwise changes in the membrane capacitance of rat peritoneal mast cells.
    J Physiol. 1987 May;386:205-17 PMID: 3681706
  7. Constitutive and regulated secretion of proteins.
    Annu Rev Cell Biol. 1987;3:243-93 PMID: 3318877
  8. GE: a GTP-binding protein mediating exocytosis.
    Annu Rev Physiol. 1990;52:591-606 PMID: 2109965
  9. Cytosolic Ca2+, exocytosis, and endocytosis in single melanotrophs of the rat pituitary.
    Neuron. 1990 Nov;5(5):723-33 PMID: 2223095
  10. Reconstitution of constitutive secretion using semi-intact cells: regulation by GTP but not calcium.
    J Cell Biol. 1991 Jan;112(1):39-54 PMID: 1986006
  11. Factors affecting dense and alpha-granule secretion from electropermeabilized human platelets: Ca(2+)-independent actions of phorbol ester and GTP gamma S.
    Cell Regul. 1990 Dec;1(13):1027-41 PMID: 1966891
  12. Calcium requirements for secretion in bovine chromaffin cells.
    J Physiol. 1992 May;450:247-71 PMID: 1432709
  13. Quantal transmitter secretion from myocytes loaded with acetylcholine.
    Nature. 1992 Oct 22;359(6397):733-6 PMID: 1436036
  14. Multiple calcium-dependent processes related to secretion in bovine chromaffin cells.
    Neuron. 1993 Jan;10(1):21-30 PMID: 8427700
  15. The membrane fusion events in degranulating guinea pig eosinophils.
    J Cell Sci. 1993 Jan;104 ( Pt 1):203-10 PMID: 8449998
  16. GTP gamma S stimulates exocytosis in patch-clamped rat melanotrophs.
    Neuron. 1993 Jul;11(1):165-72 PMID: 8338664
  17. A low affinity Ca2+ receptor controls the final steps in peptide secretion from pituitary melanotrophs.
    Neuron. 1993 Jul;11(1):93-104 PMID: 8393324
  18. Biogenesis of constitutive secretory vesicles, secretory granules and synaptic vesicles.
    Curr Opin Cell Biol. 1993 Aug;5(4):628-35 PMID: 8257604
  19. Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
    Science. 1994 Jan 21;263(5145):390-3 PMID: 7904084
  20. Two independently regulated secretory pathways in mast cells.
    J Physiol Paris. 1993;87(3):203-8 PMID: 8136786
  21. Activation of exocytosis by GTP analogues in adrenal chromaffin cells revealed by patch-clamp capacitance measurement.
    FEBS Lett. 1994 May 16;344(2-3):139-42 PMID: 8187872
  22. Tetanus toxin-mediated cleavage of cellubrevin impairs exocytosis of transferrin receptor-containing vesicles in CHO cells.
    J Cell Biol. 1994 Jun;125(5):1015-24 PMID: 8195285
  23. Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse.
    Cell. 1994 Nov 18;79(4):717-27 PMID: 7954835
  24. Kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged Ca2+.
    Biophys J. 1994 Dec;67(6):2546-57 PMID: 7696493
  25. Spontaneous quantal transmitter secretion from myocytes and fibroblasts: comparison with neuronal secretion.
    J Neurosci. 1995 Apr;15(4):2826-38 PMID: 7722632
  26. Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins.
    Nature. 1995 Jun 15;375(6532):594-9 PMID: 7791877
  27. Identification of a nonneuronal isoform of synaptotagmin.
    Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5895-9 PMID: 7597049
  28. Calcium-dependent transmitter secretion from fibroblasts: modulation by synaptotagmin I.
    Neuron. 1995 Sep;15(3):689-96 PMID: 7546747
  29. Docked granules, the exocytic burst, and the need for ATP hydrolysis in endocrine cells.
    Neuron. 1995 Nov;15(5):1085-96 PMID: 7576652
  30. Vesicle accumulation and exocytosis at sites of plasma membrane disruption.
    J Cell Biol. 1995 Dec;131(6 Pt 2):1737-45 PMID: 8557741
  31. Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6712-6 PMID: 6959149
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-08-01
Pages
3787-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452059
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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