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PMID: 8674114 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.

Cell ·Vol. 85 ·No. 7 ·1996-06-28 ·Pages 1077-88

Kiss-László Z, Henry Y, Bachellerie JP, Caizergues-Ferrer M, Kiss T

Abstract

Eukaryotic cells contain many fibrillarin-associated small nucleolar RNAs (snoRNAs) that possess long complementarities to mature rRNAs. Characterization of 21 novel antisense snoRNAs from human cells followed by genetic depletion and reconstitution studies on yeast U24 snoRNA provides evidence that this class of snoRNAs is required for site-specific 2'-O-methylation of preribosomal RNA (pre-rRNA). Antisense sno-RNAs function through direct base-pairing interactions with pre-rRNA. The antisense element, together with the D or D' box of the snoRNA, provide the information necessary to select the target nucleotide for the methyltransfer reaction. The conclusion that sno-RNAs function in covalent modification of the sugar moieties of ribonucleotides demonstrates that eukaryotic small nuclear RNAs have a more versatile cellular function than earlier anticipated.

MeSH Terms

Base Sequence Chromosomal Proteins, Non-Histone/genetics,metabolism Consensus Sequence HeLa Cells/physiology Humans Methylation Molecular Sequence Data Nucleotides/metabolism RNA Precursors/metabolism RNA, Antisense/genetics RNA, Ribosomal/metabolism RNA, Small Nuclear/metabolism,physiology Ribonucleoproteins/genetics,metabolism Ribose/genetics,metabolism Yeasts/genetics tRNA Methyltransferases/metabolism

Chemicals

Chromosomal Proteins, Non-Histone Nucleotides RNA Precursors RNA, Antisense RNA, Ribosomal RNA, Small Nuclear Ribonucleoproteins fibrillarin Ribose tRNA Methyltransferases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kiss-László Z

Laboratorie de Biologie Moléculaire Eucaryote du CNRS, Toulouse, France.

Henry Y

Bachellerie J P

Caizergues-Ferrer M

Kiss T

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(00)81308-2

Published

1996-06-28

Pages

1077-88

Language

English

Region

United States

NLM ID

0413066

Subset

Databases

GENBANK

X96640, X96641, X96642, X96643, X96644, X96645, X96646, X96647, X96648, X96649, X96650, X96651, X96652, X96653, X96654, X96655, X96656, X96657, X96658, X96659, X96660, X96661, X96662

External Links

PubMed source DOI: 10.1016/s0092-8674(00)81308-2 View journal details

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Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.

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