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PMID: 8675350 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of haemophilus influenzae type b interaction with respiratory mucosa organ cultures maintained with an air interface or immersed in medium.

Infection and immunity ·Vol. 64 ·No. 6 ·1996-06-00 ·Pages 2353-5

Jackson AD, Cole PJ, Wilson R

Abstract

Haemophilus influenzae type b infection of immersed and air interface organ cultures for 24 h caused significant epithelial damage. Bacterial association with mucus, damaged epithelium, and unciliated cells was significantly higher in air interface than immersed organ cultures, and total bacterial association was 55.8 times greater. Bacteria exhibited tropism for mucus only on explants maintained with an air interface. We conclude that immersion of nasopharyngeal tissue in medium may influence bacterial interaction with the mucosal surface.

MeSH Terms
Culture Media Haemophilus influenzae/physiology Humans Nasal Mucosa/microbiology Organ Culture Techniques
Chemicals
Culture Media
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jackson A D
Host Defence Unit, Imperial College of Science, Technology and Medicine, National Heart and Lung Institute, London, United Kingdom.
Cole P J
Wilson R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.6.2353-2355.1996
Published
1996-06-00
Pages
2353-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC174079
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/iai.64.6.2353-2355.1996 PMC Full Text View journal details
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