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PMID: 869912 Published · ppublish English Journal Article

Analysis of nucleosomal deoxyribonucleic acid in a higher plant.

The Biochemical journal ·Vol. 163 ·No. 1 ·1977-04-01 ·Pages 141-4

Cheah KS, Osborne DJ

Abstract

Comparisons of nuclease digestions of chromatin in nuclei from rye embryos and rat liver show that their nucleosomal DNA is similar, i.e. DNA subunits consist of 200 base-pair repeats with 140 base-pair cores of identical substructure. The identical nucleosome structure is present in nuclei from cells of rye embryos that have been non-viable (i.e. dead) for more than 7 years. These findings indicate a high degree of stability of the DNA-histone complex and are consistent with conservation of the nucleosomal structure of chromatin during evolution.

MeSH Terms
Base Sequence Cell Survival Chromatin/analysis DNA/analysis Edible Grain/analysis Electrophoresis, Agar Gel Secale/analysis Seeds
Chemicals
Chromatin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cheah K S
Osborne D J
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32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1630141
Published
1977-04-01
Pages
141-4
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1164669
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1630141 PMC Full Text View journal details
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