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PMID: 8699257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Localization of the somatostatin receptor SST2A in rat brain using a specific anti-peptide antibody.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 16 ·No. 14 ·1996-07-15 ·Pages 4468-78

Dournaud P, Gu YZ, Schonbrunn A, Mazella J, Tannenbaum GS, Beaudet A

Abstract

Biological actions of somatostatin are exerted via a family of receptors, for which five genes recently have been cloned. However, none of these receptor proteins has been visualized yet in the brain. In the present-study, the regional and cellular distribution of the somatostatin sst2A receptor was investigated via immunocytochemistry in the rat central nervous system by using an antibody generated against a unique sequence of the receptor protein. Specificity of the antiserum was demonstrated by immunoblot and immunocytochemistry on rat brain membranes and/or on cells transfected with cDNA encoding the different sst receptor subtypes. In rat brain sections, sst2A receptor immunoreactivity was concentrated either in perikarya and dendrites or in axon terminals distributed throughout the neuropil. Somatodendritic labeling was most prominent in the olfactory tubercle, layers II-III of the cerebral cortex, nucleus accumbens, pyramidal cells of CA1-CA2 subfields of the hippocampus, central and cortical amygdaloid nuclei, and locus coeruleus. Labeled terminals were detected mainly in the endopiriform nucleus, deep layers of the cortex, claustrum, substantia innominata, subiculum, basolateral amygdala, medial habenula, and periaqueductal gray. Electron microscopy confirmed the association of sst2A receptors with perikarya and dendrites in the former regions and with axon terminals in the latter. These results provide the first characterization of the cellular distribution of a somatostatin receptor in mammalian brain. The widespread distribution of the sst2A receptor in cerebral cortex and limbic structures suggests that it is involved in the transduction of both pre- and postsynaptic effects of somatostatin on cognition, learning, and memory.

MeSH Terms
Animals Blotting, Western Brain/immunology Female Immunohistochemistry Male Microscopy, Electron Rabbits Rats Rats, Sprague-Dawley Receptors, Somatostatin/immunology
Chemicals
Receptors, Somatostatin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dournaud P
Department of Neurology, McGill University, Montréal, Québec, Canada.
Gu Y Z
Schonbrunn A
Mazella J
Tannenbaum G S
Beaudet A
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1996-07-15
Pages
4468-78
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6578860
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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