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PMID: 8752005 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phylogenetic analysis of carbamoylphosphate synthetase genes: complex evolutionary history includes an internal duplication within a gene which can root the tree of life.

Molecular biology and evolution ·Vol. 13 ·No. 7 ·1996-09-00 ·Pages 970-7

Lawson FS, Charlebois RL, Dillon JA

Abstract

Carbamoylphosphate synthetase (CPS) catalyzes the first committed step in pyrimidine biosynthesis, arginine biosynthesis, or the urea cycle. Organisms may contain either one generalized or two specific CPS enzymes, and these enzymes may be heterodimeric (encoded by linked or unlinked genes), monomeric, or part of a multifunctional protein. In order to help elucidate the evolution of CPS, we have performed a comprehensive phylogenetic analysis using the 21 available complete CPS sequences, including a sequence from Sulfolobus solfataricus P2 which we report in this paper. This is the first report of a complete CPS gene sequence from an archaeon, and sequence analysis suggests that it encodes an enzyme similar to heterodimeric CPSII. We confirm that internal similarity within the synthetase domain of CPS is the result of an ancient gene duplication that preceded the divergence of the Bacteria, Archaea, and Eukarya, and use this internal duplication in phylogenetic tree construction to root the tree of life. Our analysis indicates with high confidence that this archaeal sequence is more closely related to those of Eukarya than to those of Bacteria. In addition to this ancient duplication which created the synthetase domain, our phylogenetic analysis reveals a complex history of further gene duplications, fusions, and other events which have played an integral part in the evolution of CPS.

MeSH Terms

Amino Acid Sequence Animals Bacterial Proteins/genetics Carbamoyl-Phosphate Synthase (Ammonia)/genetics Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/genetics Carbon-Nitrogen Ligases Cloning, Molecular Eukaryotic Cells/physiology Evolution, Molecular Gram-Positive Bacteria/genetics Humans Ligases/genetics Models, Biological Models, Genetic Molecular Sequence Data Multigene Family Phylogeny Sulfolobus/enzymology,genetics

Chemicals

Bacterial Proteins Ligases Carbon-Nitrogen Ligases carbamoyl-phosphate synthetase (N-acetylglutamate) Carbamoyl-Phosphate Synthase (Ammonia) Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lawson F S

Department of Biology, University of Ottawa, Ontario, Canada.

Charlebois R L

Dillon J A

Article Info

Journal

Molecular biology and evolution

Abbr.

Mol Biol Evol

ISSN

0737-4038

DOI

10.1093/oxfordjournals.molbev.a025665

Published

1996-09-00

Pages

970-7

Language

English

Region

United States

NLM ID

8501455

Subset

Databases

GENBANK

J01597, J05503, J05512, K01178, K02132, L08965, L31362, L32150, M11710, M12318, M12319, M12320, M12321, M12322, M12324, M12325, M27174, M59757, U04992, U04993, U05193, U11295, U18792, U33768, X13200, X14533, X55433, X73308, Z26919

External Links

PubMed source DOI: 10.1093/oxfordjournals.molbev.a025665 View journal details

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Phylogenetic analysis of carbamoylphosphate synthetase genes: complex evolutionary history includes an internal duplication within a gene which can root the tree of life.

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