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PMID: 8760829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ligation of CD40 on dendritic cells triggers production of high levels of interleukin-12 and enhances T cell stimulatory capacity: T-T help via APC activation.

The Journal of experimental medicine ·Vol. 184 ·No. 2 ·1996-08-01 ·Pages 747-52

Cella M, Scheidegger D, Palmer-Lehmann K, Lane P, Lanzavecchia A, Alber G

Abstract

We investigated the possibility that T helper cells might enhance the stimulatory function of dendritic cells (DCs). We found that ligation of CD40 by CD40L triggers the production of extremely high levels of bioactive IL-12. Other stimuli such as microbial agents, TNF-alpha or LPS are much less effective or not at all. In addition, CD40L is the most potent stimulus in upregulating the expression of ICAM-1, CD80, and CD86 molecules on DCs. These effects of CD40 ligation result in an increased capacity of DCs to trigger proliferative responses and IFN-gamma production by T cells. These findings reveal a new role for CD40-CD40L interaction in regulating DC function and are relevant to design therapeutic strategies using cultured DCs.

MeSH Terms
Antigen-Presenting Cells/immunology Antigens, CD/metabolism B7-1 Antigen/metabolism B7-2 Antigen CD40 Antigens/physiology CD40 Ligand Cell Adhesion Cells, Cultured Dendritic Cells/immunology Fluorescent Antibody Technique, Indirect Histocompatibility Antigens Class II/metabolism Humans Intercellular Adhesion Molecule-1/metabolism Interferon-gamma/biosynthesis Interleukin-12/biosynthesis Lymphocyte Activation Lymphocyte Cooperation Membrane Glycoproteins/metabolism,physiology Signal Transduction T-Lymphocytes/immunology
Chemicals
Antigens, CD B7-1 Antigen B7-2 Antigen CD40 Antigens CD86 protein, human Histocompatibility Antigens Class II Membrane Glycoproteins Intercellular Adhesion Molecule-1 CD40 Ligand Interleukin-12 Interferon-gamma
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cella M
Basel Institute for Immunology, Switzerland.
Scheidegger D
Palmer-Lehmann K
Lane P
Lanzavecchia A
Alber G
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.2.747
Published
1996-08-01
Pages
747-52
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192696
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.184.2.747 PMC Full Text View journal details
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