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PMID: 8762062 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chloride channel blockers decrease intracellular pH in cultured renal epithelial LLC-PK1 cells.

British journal of pharmacology ·Vol. 118 ·No. 3 ·1996-06-00 ·Pages 443-4

Brown CD, Dudley AJ

Abstract

The effects of chloride channel blockers upon intracellular pH (pHi) were examined in renal epithelial monolayers of LLC-PK1 cells. A significant intracellular acidification was found with addition of 100 microM 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), niflumic acid, flufenamate and diphenylamine-2-carboxylate (DPC) but not with 4,4'-diisothiocyanatostilbene-2-2'disulphonic acid (DIDS). The effects of these agents upon pHi was dose-dependent with apparent K0.5 values of: 16.7 +/- 0.3 microM, 34.2 +/- 0.9 microM and 740 +/- 13 microM for niflumic acid, flufenamate and DPC respectively. The results indicate that at concentrations commonly used to block channel activity these chloride channel blockers have profound effects upon pHi.

MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology Animals Chloride Channels/antagonists & inhibitors Dose-Response Relationship, Drug Epithelium/drug effects Hydrogen-Ion Concentration/drug effects LLC-PK1 Cells Niflumic Acid/pharmacology Swine Time Factors
Chemicals
Chloride Channels Niflumic Acid 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown C D
Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne.
Dudley A J
References (8)
8 references, click to expand
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1996.tb15422.x
Published
1996-06-00
Pages
443-4
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1909703
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1996.tb15422.x PMC Full Text View journal details
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