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PMID: 8764073 Published · ppublish English Journal Article

A putative receptor for Venezuelan equine encephalitis virus from mosquito cells.

Journal of virology ·Vol. 70 ·No. 8 ·1996-08-00 ·Pages 5592-9

Ludwig GV, Kondig JP, Smith JF

Abstract

We have identified a cellular protein from a continuous mosquito cell line (C6/36) that appears to play a significant role in the attachment of Venezuelan equine encephalitis (VEE) virus to these cells. VEE virus bound to a 32-kDa polypeptide present in the C6/36 plasma membrane fraction, and binding to this polypeptide was dose dependent and saturable and competed with homologous and heterologous alphaviruses. These observations suggest that this polypeptide binds virus via a receptor-ligand interaction. The 32-kDa polypeptide was expressed on the surfaces of C6/36 cells, and monoclonal antibodies directed against either this cell polypeptide or the VEE virus E2 glycoprotein, which is thought to be the viral attachment protein, interfered with virus attachment. Collectively, these data provide evidence suggesting that the 32-kDa polypeptide serves as a receptor for VEE virus infection of cells. We have characterized this cell polypeptide as a laminin-binding protein on the basis of its ability to interact directly with laminin as well as its immunologic cross-reactivity with the high-affinity human laminin receptor.

MeSH Terms
Animals Antibodies, Monoclonal Cell Line Culicidae/virology Encephalitis Virus, Venezuelan Equine/physiology Humans Molecular Weight Peptides/analysis,immunology,isolation & purification Receptors, Virus/analysis,immunology,isolation & purification
Chemicals
Antibodies, Monoclonal Peptides Receptors, Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ludwig G V
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA.
Kondig J P
Smith J F
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.8.5592-5599.1996
Published
1996-08-00
Pages
5592-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190519
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.8.5592-5599.1996 PMC Full Text View journal details
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