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PMID: 8770871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of scavenger receptor, class B, type I, a high density lipoprotein receptor, in liver and steroidogenic tissues of the rat.

The Journal of clinical investigation ·Vol. 98 ·No. 4 ·1996-08-15 ·Pages 984-95

Landschulz KT, Pathak RK, Rigotti A, Krieger M, Hobbs HH

Abstract

The scavenger receptor, class B, type I (SR-BI) binds HDL and mediates the selective transfer of cholesteryl esters from HDL to cultured cells. The tissue distribution of SR-BI in mice suggests that this receptor may deliver HDL-cholesterol to the liver and to nonplacental steroidogenic tissues. To examine the role of SR-BI in vivo, we determined its tissue and cell type-specific expression pattern and regulation in rats. High levels of immunodetectable SR-BI were present in the adrenal gland, ovary, and liver. In pregnant animals, the mammary gland also expressed high levels of the protein. SR-BI was localized by immunofluorescence to the surfaces of steroidogenic cells in the zona fasciculata and zona reticularis of the adrenal gland and to the corpus luteal cells of the ovary. High-dose estrogen treatment dramatically reduced SR-BI in the liver and increased SR-BI in the adrenal gland and corpus luteal cells of the ovary. These estrogen-induced increases in SR-BI in the adrenal gland and ovary were accompanied by enhanced in vivo uptake of fluorescent lipid from HDL. The administration of human chorionic gonadotropin induced a dramatic increase in SR-BI in the steroidogenic Leydig cells of the testes. These findings suggest that SR-BI mediates physiologically relevant uptake of cholesterol from HDL to nonplacental steroidogenic tissues in vivo.

MeSH Terms
Adrenal Glands/metabolism Animals Apolipoprotein A-I/metabolism Apolipoproteins E/metabolism Base Sequence Blotting, Western CD36 Antigens/metabolism Carrier Proteins Cholesterol/metabolism Cholesterol, HDL/metabolism Chorionic Gonadotropin/pharmacology Ethinyl Estradiol/pharmacology Female Fluorescent Antibody Technique, Indirect Gene Expression Lipoproteins, HDL Liver/enzymology Male Membrane Proteins Molecular Sequence Data Oligonucleotide Probes/chemistry Ovary/metabolism Pregnancy RNA, Messenger/genetics RNA-Binding Proteins Rats Rats, Sprague-Dawley Receptors, Immunologic/metabolism Receptors, LDL/metabolism Receptors, Lipoprotein/metabolism Receptors, Scavenger Scavenger Receptors, Class B Steroids/biosynthesis Testis/metabolism Tissue Distribution
Chemicals
Apolipoprotein A-I Apolipoproteins E CD36 Antigens Carrier Proteins Cholesterol, HDL Chorionic Gonadotropin Lipoproteins, HDL Membrane Proteins Oligonucleotide Probes RNA, Messenger RNA-Binding Proteins Receptors, Immunologic Receptors, LDL Receptors, Lipoprotein Receptors, Scavenger Scarb1 protein, mouse Scarb1 protein, rat Scavenger Receptors, Class B Steroids high density lipoprotein receptors high density lipoprotein binding protein Ethinyl Estradiol Cholesterol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Landschulz K T
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235, USA.
Pathak R K
Rigotti A
Krieger M
Hobbs H H
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI118883
Published
1996-08-15
Pages
984-95
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC507514
Subset
IM
Grants
NHLBI NIH HHS · HL-20948 · United States
NHLBI NIH HHS · HL41484 · United States
NHLBI NIH HHS · HL52212 · United States
External Links
PubMed source DOI: 10.1172/JCI118883 PMC Full Text View journal details
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