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PMID: 8774892 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS.

Nucleic acids research ·Vol. 24 ·No. 16 ·1996-08-15 ·Pages 3134-41

Riechmann JL, Wang M, Meyerowitz EM

Abstract

The MADS domain proteins APETALA1 (AP1), APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG) specify the identity of Arabidopsis floral organs. AP1 and AG homocomplexes and AP3-PI heterocomplexes bind to CArG-box sequences. The DNA-binding properties of these complexes were investigated. We find that AP1, AG and AP3-PI are all capable of recognizing the same DNA-binding sites, although with somewhat different affinities. In addition, the three complexes induce similar conformational changes on a CArG-box sequence. Phasing analysis reveals that the induced distortion is DNA bending, oriented toward the minor groove. The molecular dissection of AP1, AP3, PI and AG indicates that the boundaries of the dimerization domains of these proteins vary. The regions required to form a DNA-binding complex include, in addition to the MADS box, the entire L region (which follows the MADS box) and the first putative amphipathic helix of the K box in the case of AP3-PI, while for AP1 and AG only a part of the L region is needed. The similarity of the DNA-binding properties of AP1, AP3-PI and AG is discussed with regard to the biological specificity that these proteins exhibit.

MeSH Terms
AGAMOUS Protein, Arabidopsis Arabidopsis/genetics Arabidopsis Proteins Base Sequence Binding Sites DNA Probes/metabolism DNA-Binding Proteins/genetics,metabolism Homeodomain Proteins/metabolism MADS Domain Proteins Molecular Sequence Data Nucleic Acid Conformation Plant Proteins/metabolism Protein Binding Recombinant Proteins/metabolism Transcription Factors/genetics,metabolism
Chemicals
AGAMOUS Protein, Arabidopsis AP1 protein, Arabidopsis APETALA 3 protein, Arabidopsis Arabidopsis Proteins DNA Probes DNA-Binding Proteins Homeodomain Proteins MADS Domain Proteins PISTILLATA protein, Arabidopsis Plant Proteins Recombinant Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Riechmann J L
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Wang M
Meyerowitz E M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.16.3134
Published
1996-08-15
Pages
3134-41
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146081
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/24.16.3134 PMC Full Text View journal details
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