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PMID: 8781233 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Small-conductance, calcium-activated potassium channels from mammalian brain.

Science (New York, N.Y.) ·Vol. 273 ·No. 5282 ·1996-09-20 ·Pages 1709-14

Köhler M, Hirschberg B, Bond CT, Kinzie JM, Marrion NV, Maylie J, Adelman JP

Abstract

Members of a previously unidentified family of potassium channel subunits were cloned from rat and human brain. The messenger RNAs encoding these subunits were widely expressed in brain with distinct yet overlapping patterns, as well as in several peripheral tissues. Expression of the messenger RNAs in Xenopus oocytes resulted in calcium-activated, voltage-independent potassium channels. The channels that formed from the various subunits displayed differential sensitivity to apamin and tubocurare. The distribution, function, and pharmacology of these channels are consistent with the SK class of small-conductance, calcium-activated potassium channels, which contribute to the afterhyperpolarization in central neurons and other cell types.

MeSH Terms

Amino Acid Sequence Animals Antisense Elements (Genetics) Apamin/pharmacology Brain Chemistry Calcium/metabolism,pharmacology Cloning, Molecular Electric Conductivity Female Humans Membrane Potentials Molecular Sequence Data Neurons/physiology Oocytes Patch-Clamp Techniques Potassium/metabolism Potassium Channel Blockers Potassium Channels/analysis,chemistry,physiology Potassium Channels, Calcium-Activated RNA, Messenger/analysis,genetics Rats Rats, Sprague-Dawley Small-Conductance Calcium-Activated Potassium Channels Xenopus

Chemicals

Antisense Elements (Genetics) Potassium Channel Blockers Potassium Channels Potassium Channels, Calcium-Activated RNA, Messenger Small-Conductance Calcium-Activated Potassium Channels Apamin Potassium Calcium

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Köhler M

Vollum Institute, L-474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Road, Portland, OR 97201, USA. J. Maylie, Department of Obstetrics and Gyne.

Hirschberg B

Bond C T

Kinzie J M

Marrion N V

Maylie J

Adelman J P

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.273.5282.1709

Published

1996-09-20

Pages

1709-14

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

D42145, L02750, L16912, M64226, M64228, U01071, U11717, U69882, U69883, U69884, U69885, X60796, X72341, X73052, X83581, X83585

Corrections

CommentIn

PMID: 8830412

External Links

PubMed source DOI: 10.1126/science.273.5282.1709 View journal details

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Small-conductance, calcium-activated potassium channels from mammalian brain.

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