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PMID: 8790442 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Stable transfer of intact high molecular weight DNA into plant chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 18 ·1996-09-03 ·Pages 9975-9

Hamilton CM, Frary A, Lewis C, Tanksley SD

Abstract

In conjunction with an enhanced system for Agrobacterium-mediated plant transformation, a new binary bacterial artificial chromosome (BIBAC) vector has been developed that is capable of transferring at least 150 kb of foreign DNA into a plant nuclear genome. The transferred DNA appears to be intact in the majority of transformed tobacco plants analyzed and is faithfully inherited in the progeny. The ability to introduce high molecular weight DNA into plant chromosomes should accelerate gene identification and genetic engineering of plants and may lead to new approaches in studies of genome organization.

MeSH Terms
Base Sequence DNA, Bacterial/genetics DNA, Plant/chemistry,metabolism Gene Transfer Techniques Molecular Sequence Data Restriction Mapping Rhizobium
Chemicals
DNA, Bacterial DNA, Plant T-DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hamilton C M
Plant Science Center, Cornell University, Ithaca, NY 14853, USA.
Frary A
Lewis C
Tanksley S D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.18.9975
Published
1996-09-03
Pages
9975-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38540
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.18.9975 PMC Full Text View journal details
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