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PMID: 8799137 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Interleaflet clear space is reduced in the membrane of COP I and COP II-coated buds/vesicles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 17 ·1996-08-20 ·Pages 8968-70

Orci L, Schekman R, Perrelet A

Abstract

Intracellular transfers between membrane-bound compartments occur through vesicles that bud from a donor compartment to fuse subsequently with an acceptor membrane. We report that the membrane that delimits COP I or COP II-coated buds/vesicles from the endoplasmic reticulum and the Golgi complex has a thinner interleaflet clear space as compared with the surrounding, noncoated parental membrane. This change is compatible with a compositional change of the membrane bilayer during the budding process.

MeSH Terms
COP-Coated Vesicles Coated Vesicles/chemistry,ultrastructure Cryoultramicrotomy Densitometry Endoplasmic Reticulum/chemistry,ultrastructure Fungal Proteins/isolation & purification GTP-Binding Proteins/isolation & purification GTPase-Activating Proteins Golgi Apparatus/chemistry,ultrastructure Membrane Proteins/isolation & purification Microscopy, Immunoelectron Monomeric GTP-Binding Proteins Nuclear Pore Complex Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins
Chemicals
Fungal Proteins GTPase-Activating Proteins Membrane Proteins Nuclear Pore Complex Proteins SEC13 protein, S cerevisiae SEC23 protein, S cerevisiae Saccharomyces cerevisiae Proteins Vesicular Transport Proteins GTP-Binding Proteins Monomeric GTP-Binding Proteins SAR1 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Orci L
Department of Morphology, University of Geneva Medical Center, Switzerland.
Schekman R
Perrelet A
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18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.17.8968
Published
1996-08-20
Pages
8968-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38578
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.17.8968 PMC Full Text View journal details
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