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PMID: 8799142 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Poly (alpha 2,8-deaminoneuraminic acid) is expressed in lung on a single 150-kDa glycoprotein and is an oncodevelopmental antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 17 ·1996-08-20 ·Pages 8995-8

Qu B, Ziak M, Zuber C, Roth J

Abstract

Homopolymers of alpha 2,8-linked N-acetylneuraminic acid [poly(alpha 2,8-Neu5Ac)] of the neural cell adhesion molecule NCAM have been shown to be temporally expressed during lung development and represent a marker for small cell lung carcinoma. We report the presence of a further polysialic acid in lung that consists of oligo/polymers of alpha 2,8-linked deaminoneuraminic acid residues [poly (alpha 2,8-KDN)], as detected with a monoclonal antibody in conjunction with a specific sialidase. Although the various cell types forming the bronchi, alveolar septs, and blood vessels were positive for poly (alpha 2,8-KDN) by immunohistochemistry, this polysialic acid was found on a single 150-kDa glycoprotein by immunoblot analysis. The poly(alpha 2,8-KDN)-bearing glycoprotein was not related to an NCAM protein based on immunochemical criteria. The expression of the poly (alpha 2,8-KDN) was developmentally regulated as evidenced by its gradual disappearance in the rat lung parenchyma commencing 1 week after birth. In adult lung the blood vessel endothelia and the smooth muscle fibers of both blood vessels and bronchi were positive but not the bronchial and alveolar epithelium. The poly (alpha 2,8-KDN)-bearing 150-kDa glycoprotein became reexpressed in various histological types of lung carcinomas and cell lines derived from them and represents a new oncodevelopmental antigen in lung.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Neoplasm/immunology,isolation & purification Biomarkers, Tumor Carcinoma/chemistry Glycoproteins/immunology,isolation & purification Glycoside Hydrolases/metabolism Humans Immunohistochemistry Lung/chemistry,growth & development Lung Neoplasms/chemistry Polysaccharides/immunology,isolation & purification Rats
Chemicals
Antibodies, Monoclonal Antigens, Neoplasm Biomarkers, Tumor Glycoproteins Polysaccharides poly(2,8-deaminoneuraminic acid) Glycoside Hydrolases deaminoneuraminate hydrolase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Qu B
Department of Pathology, University of Zürich, Switzerland.
Ziak M
Zuber C
Roth J
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.17.8995
Published
1996-08-20
Pages
8995-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38583
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.17.8995 PMC Full Text View journal details
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