Home LiteratureArticle Details
PMID: 8799178 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning, expression, and distribution of a Ca(2+)-activated K+ channel beta-subunit from human brain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 17 ·1996-08-20 ·Pages 9200-5

Tseng-Crank J, Godinot N, Johansen TE, Ahring PK, Strøbaek D, Mertz R, Foster CD, Olesen SP, Reinhart PH

Abstract

We have cloned and expressed a Ca(2+)-activated K+ channel beta-subunit from human brain. The open reading frame encodes a 191-amino acid protein possessing significant homology to a previously described subunit cloned from bovine muscle. The gene for this subunit is located on chromosome 5 at band q34 (hslo-beta). There is no evidence for alternative RNA splicing of this gene product. hslo-beta mRNA is abundantly expressed in smooth muscle, but expression levels are low in most other tissues, including brain. Brain subregions in which beta-subunit mRNA expression is relatively high are the hippocampus and corpus callosum. The coexpression of hslo-beta mRNA together with hslo-alpha subunits in either Xenopus oocytes or stably transfected HEK 293 cells give rise to Ca(2+)-activated potassium currents with a much increased calcium and/or voltage sensitivity. These data indicate that the beta-subunit shows a tissue distribution different to that of the alpha-subunit, and in many tissues there may be no association of alpha-subunits with beta-subunits. These beta-subunits can play a functional role in the regulation of neuronal excitability by tuning the Ca2+ and/or the voltage dependence of alpha-subunits.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain Chemistry/genetics Calcium/pharmacology Cattle Chromosome Mapping Chromosomes, Human, Pair 5/genetics Cloning, Molecular Corpus Callosum/chemistry Hippocampus/chemistry Humans In Situ Hybridization, Fluorescence Ion Channel Gating Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Large-Conductance Calcium-Activated Potassium Channel beta Subunits Large-Conductance Calcium-Activated Potassium Channels Molecular Sequence Data Patch-Clamp Techniques Potassium Channels/biosynthesis,drug effects,genetics Potassium Channels, Calcium-Activated RNA, Messenger/isolation & purification Recombinant Proteins/biosynthesis Sequence Homology, Amino Acid Species Specificity Tissue Distribution Xenopus
Chemicals
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Large-Conductance Calcium-Activated Potassium Channel beta Subunits Large-Conductance Calcium-Activated Potassium Channels Potassium Channels Potassium Channels, Calcium-Activated RNA, Messenger Recombinant Proteins Calcium
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Tseng-Crank J
Glaxo-Wellcome Research Institute, Research Triangle Park, NC 27709, USA.
Godinot N
Johansen T E
Ahring P K
Strøbaek D
Mertz R
Foster C D
Olesen S P
Reinhart P H
References (40)
40 references, click to expand
  1. Gating kinetics of Ca2+-activated K+ channels from rat muscle incorporated into planar lipid bilayers. Evidence for two voltage-dependent Ca2+ binding reactions.
    J Gen Physiol. 1983 Oct;82(4):511-42 PMID: 6315857
  2. Quantification of Ca2+-activated K+ channels under hormonal control in pig pancreas acinar cells.
    Nature. 1983 Sep 15-21;305(5931):228-32 PMID: 6310413
  3. Potassium channels in cultured bovine adrenal chromaffin cells.
    J Physiol. 1985 Oct;367:117-41 PMID: 2414437
  4. Varieties of calcium-activated potassium channels.
    Annu Rev Physiol. 1989;51:385-99 PMID: 2653189
  5. A family of calcium-dependent potassium channels from rat brain.
    Neuron. 1989 Jan;2(1):1031-41 PMID: 2624739
  6. Heteropolymeric potassium channels expressed in Xenopus oocytes from cloned subunits.
    Neuron. 1990 Mar;4(3):405-11 PMID: 2317379
  7. Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes.
    Nature. 1990 Jun 7;345(6275):530-4 PMID: 2112229
  8. Shaker K+ channel subunits from heteromultimeric channels with novel functional properties.
    Biochem Biophys Res Commun. 1990 Sep 28;171(3):1361-71 PMID: 1699527
  9. Colocalization of ion channels involved in frequency selectivity and synaptic transmission at presynaptic active zones of hair cells.
    J Neurosci. 1990 Nov;10(11):3664-84 PMID: 1700083
  10. Modulation of calcium-activated potassium channels from rat brain by protein kinase A and phosphatase 2A.
    J Neurosci. 1991 Jun;11(6):1627-35 PMID: 1646298
  11. A component of calcium-activated potassium channels encoded by the Drosophila slo locus.
    Science. 1991 Aug 2;253(5019):551-5 PMID: 1857984
  12. Protein kinase activity closely associated with a reconstituted calcium-activated potassium channel.
    Science. 1991 Aug 2;253(5019):560-2 PMID: 1857986
  13. Calcium-activated potassium channels: regulation by calcium.
    J Bioenerg Biomembr. 1991 Aug;23(4):537-60 PMID: 1917908
  14. Presynaptic calcium signals and transmitter release are modulated by calcium-activated potassium channels.
    J Neurosci. 1992 Jan;12(1):297-305 PMID: 1370323
  15. Calcium-binding sites in proteins: a structural perspective.
    Adv Protein Chem. 1991;42:77-144 PMID: 1793008
  16. Chromosomal localization of FLT4, a novel receptor-type tyrosine kinase gene.
    Genomics. 1992 Jun;13(2):475-8 PMID: 1319394
  17. Calcium-activated potassium channels expressed from cloned complementary DNAs.
    Neuron. 1992 Aug;9(2):209-16 PMID: 1497890
  18. A mouse genomic library in the bacteriophage P1 cloning system: organization and characterization.
    Mamm Genome. 1992;3(10):550-8 PMID: 1421762
  19. Colocalization of active KCa channels and Ca2+ channels within Ca2+ domains in helix neurons.
    Neuron. 1993 Apr;10(4):689-99 PMID: 8386529
  20. mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels.
    Science. 1993 Jul 9;261(5118):221-4 PMID: 7687074
  21. Functional colocalization of calcium and calcium-gated potassium channels in control of transmitter release.
    Neuron. 1993 Oct;11(4):645-55 PMID: 7691106
  22. Purification and reconstitution of the high-conductance, calcium-activated potassium channel from tracheal smooth muscle.
    J Biol Chem. 1994 Jan 7;269(1):676-82 PMID: 7506261
  23. Subunit composition of the high conductance calcium-activated potassium channel from smooth muscle, a representative of the mSlo and slowpoke family of potassium channels.
    J Biol Chem. 1994 Feb 11;269(6):3921-4 PMID: 7508434
  24. Functional dependence of Ca(2+)-activated K+ current on L- and N-type Ca2+ channels: differences between chicken sympathetic and parasympathetic neurons suggest different regulatory mechanisms.
    Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2858-62 PMID: 8146200
  25. Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
    Nature. 1994 May 26;369(6478):289-94 PMID: 8183366
  26. Primary sequence and immunological characterization of beta-subunit of high conductance Ca(2+)-activated K+ channel from smooth muscle.
    J Biol Chem. 1994 Jun 24;269(25):17274-8 PMID: 8006036
  27. Auxiliary subunits of voltage-gated ion channels.
    Neuron. 1994 Jun;12(6):1183-94 PMID: 7516685
  28. Further evidence for clustering of human GABAA receptor subunit genes: localization of the alpha 6-subunit gene (GABRA6) to distal chromosome 5q by linkage analysis.
    Genomics. 1994 Mar 15;20(2):285-8 PMID: 8020978
  29. Phosphorylation and dephosphorylation modulate a Ca(2+)-activated K+ channel in rat peptidergic nerve terminals.
    J Physiol. 1994 Mar 1;475(2):241-54 PMID: 8021831
  30. Reconstitution of expressed KCa channels from Xenopus oocytes to lipid bilayers.
    Biophys J. 1994 Apr;66(4):1022-7 PMID: 7518702
  31. Cloning, expression, and distribution of functionally distinct Ca(2+)-activated K+ channel isoforms from human brain.
    Neuron. 1994 Dec;13(6):1315-30 PMID: 7993625
  32. A novel beta subunit increases rate of inactivation of specific voltage-gated potassium channel alpha subunits.
    J Biol Chem. 1995 Mar 17;270(11):6272-7 PMID: 7890764
  33. Functional role of the beta subunit of high conductance calcium-activated potassium channels.
    Neuron. 1995 Mar;14(3):645-50 PMID: 7695911
  34. Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro.
    J Neurosci. 1995 May;15(5 Pt 2):3840-51 PMID: 7751950
  35. Kinase and phosphatase activities intimately associated with a reconstituted calcium-dependent potassium channel.
    J Neurosci. 1995 Jun;15(6):4572-9 PMID: 7790924
  36. Association and colocalization of K+ channel alpha- and beta-subunit polypeptides in rat brain.
    J Neurosci. 1995 Jul;15(7 Pt 2):5360-71 PMID: 7623158
  37. Distinct effects of Ca2+ and voltage on the activation and deactivation of cloned Ca(2+)-activated K+ channels.
    J Physiol. 1995 Dec 1;489 ( Pt 2):403-18 PMID: 8847636
  38. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
    Pflugers Arch. 1981 Aug;391(2):85-100 PMID: 6270629
  39. A calcium-dependent transient outward current in Xenopus laevis oocytes.
    Proc R Soc Lond B Biol Sci. 1982 Jul 22;215(1201):491-7 PMID: 6127718
  40. The contribution of both extracellular and intracellular calcium to the action of alpha-adrenergic agonists in perfused rat liver.
    Biochem J. 1984 May 15;220(1):35-42 PMID: 6743271
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.17.9200
Published
1996-08-20
Pages
9200-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38619
Subset
IM
Grants
NINDS NIH HHS · NS31253 · United States
Databases
GENBANK
U42600, U42601, U42602, U42603
External Links
PubMed source DOI: 10.1073/pnas.93.17.9200 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2