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PMID: 8808922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Candida albicans HYR1 gene, which is activated in response to hyphal development, belongs to a gene family encoding yeast cell wall proteins.

Journal of bacteriology ·Vol. 178 ·No. 18 ·1996-09-00 ·Pages 5353-60

Bailey DA, Feldmann PJ, Bovey M, Gow NA, Brown AJ

Abstract

A hyphally regulated gene (HYR1) from the dimorphic human pathogenic fungus Candida albicans was isolated and characterized. Northern (RNA) analyses showed that the HYR1 mRNA was induced specifically in response to hyphal development when morphogenesis was stimulated by serum addition and temperature elevation, increases in both culture pH and temperature, or N-acetylglucosamine addition. The HYR1 gene sequence revealed a 937-codon open reading frame capable of encoding a protein with an N-terminal signal sequence, a C-terminal glycosylphosphatidylinositol-anchoring domain, 17 potential N glycosylation sites, and a large domain rich in serine and threonine (51% of 230 residues). These features are observed in many yeast cell wall proteins, but no homologs are present in the databases. In addition, Hyr1p contained a second domain rich in glycine, serine, and asparagine (79% of 239 residues). The HYR1 locus in C. albicans CAI4 was disrupted by "Ura-blasting," but the resulting homozygous delta hyr1/delta hyr1 null mutant displayed no obvious morphological phenotype. The growth rates for yeast cells and hyphae and the kinetics of germ tube formation in the null mutant were unaffected. Aberrant expression of HYR1 in yeast cells, when an ADH1-HYR1 fusion was used, did not stimulate hyphal formation in C. albicans or pseudohyphal growth in Saccharomyces cerevisiae. HYR1 appears to encode a nonessential component of the hyphal cell wall.

MeSH Terms
Amino Acid Sequence Base Sequence Candida albicans/genetics,growth & development Cell Wall/chemistry Chromosome Mapping DNA, Complementary/genetics Fungal Proteins/genetics Gene Expression Regulation, Fungal Genes, Fungal Genomic Library Glycosylphosphatidylinositols Molecular Sequence Data Morphogenesis/genetics Mutagenesis Protein Sorting Signals Saccharomyces cerevisiae/genetics,metabolism Sequence Analysis, DNA Transcription, Genetic
Chemicals
DNA, Complementary Fungal Proteins Glycosylphosphatidylinositols HYR1 protein, Candida albicans Protein Sorting Signals
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bailey D A
Department of Molecular and Cell Biology, University of Aberdeen, United Kingdom.
Feldmann P J
Bovey M
Gow N A
Brown A J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.18.5353-5360.1996
Published
1996-09-00
Pages
5353-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178351
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
Z50123
External Links
PubMed source DOI: 10.1128/jb.178.18.5353-5360.1996 PMC Full Text View journal details
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