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PMID: 8816999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Overexpression of cytoplasmic dynein's globular head causes a collapse of the interphase microtubule network in Dictyostelium.

Molecular biology of the cell ·Vol. 7 ·No. 6 ·1996-06-00 ·Pages 935-48

Koonce MP, Samsó M

Abstract

Cytoplasmic dynein is a minus-end directed microtubule-based motor. Using a molecular genetic approach, we have begun to dissect structure-function relationships of dynein in the cellular slime mold Dictyostelium. Expression of a carboxy-terminal 380-kDa fragment of the heavy chain produces a protein that approximates the size and shape of the globular, mechanochemical head of dynein. This polypeptide cosediments with microtubules in an ATP-sensitive fashion and undergoes a UV-vanadate cleavage reaction. The deleted amino-terminal region appears to participate in dimerization of the native protein and in binding the intermediate and light chains. Overexpression of the 380-kDa carboxy-terminal construct in Dictyostelium produces a distinct phenotype in which the interphase radial microtubule array appears collapsed. In many cells, the microtubules form loose bundles that are whorled around the nucleus. Similar expression of a central 107-kDa fragment of the heavy chain does not produce this result. The data presented here suggest that dynein may participate in maintaining the spatial pattern of the interphase microtubule network.

MeSH Terms
Animals Cytoplasm/enzymology Cytoskeleton/ultrastructure Dictyostelium/ultrastructure Dyneins/chemistry,physiology Interphase/drug effects Microscopy, Electron Microtubules/drug effects,genetics Molecular Structure Phenotype Structure-Activity Relationship
Chemicals
Dyneins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Koonce M P
Division of Molecular Medicine, Wadsworth Center, Albany, New York 12201-0509, USA.
Samsó M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.7.6.935
Published
1996-06-00
Pages
935-48
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275944
Subset
IM
Grants
NIGMS NIH HHS · GM-29169 · United States
NIGMS NIH HHS · GM-51532 · United States
External Links
PubMed source DOI: 10.1091/mbc.7.6.935 PMC Full Text View journal details
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