Home LiteratureArticle Details
PMID: 8834903 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.

Antimicrobial agents and chemotherapy ·Vol. 40 ·No. 2 ·1996-02-00 ·Pages 477-80

Versalovic J, Shortridge D, Kibler K, Griffy MV, Beyer J, Flamm RK, Tanaka SK, Graham DY, Go MF

Abstract

Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of 23S rRNA. A-->G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.

MeSH Terms
Anti-Bacterial Agents/pharmacology Base Sequence Clarithromycin/pharmacology Drug Resistance, Microbial Electrophoresis, Agar Gel Helicobacter pylori/drug effects,genetics,isolation & purification Microbial Sensitivity Tests Molecular Sequence Data Mutation Protein Synthesis Inhibitors/pharmacology RNA, Ribosomal, 23S/drug effects,genetics Sequence Homology, Nucleic Acid
Chemicals
Anti-Bacterial Agents Protein Synthesis Inhibitors RNA, Ribosomal, 23S Clarithromycin
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Versalovic J
Division of Digestive Diseases, Baylor College of Medicine, Houston, Texas, USA. [email protected]
Shortridge D
Kibler K
Griffy M V
Beyer J
Flamm R K
Tanaka S K
Graham D Y
Go M F
References (21)
21 references, click to expand
  1. Identification of two erythromycin resistance mutations in the mitochondrial gene coding for the large ribosomal RNA in yeast.
    Nucleic Acids Res. 1982 Nov 11;10(21):6571-7 PMID: 6757866
  2. Eradication of Helicobacter pylori with clarithromycin and omeprazole.
    Gut. 1994 Mar;35(3):323-6 PMID: 8150340
  3. Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coli.
    J Bacteriol. 1985 May;162(2):551-7 PMID: 3886627
  4. Functional consequences of binding macrolides to ribosomes.
    J Antimicrob Chemother. 1985 Jul;16 Suppl A:23-34 PMID: 3932309
  5. Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA.
    Biochimie. 1987 Aug;69(8):879-84 PMID: 3122849
  6. A plasmid-coded and site-directed mutation in Escherichia coli 23S RNA that confers resistance to erythromycin: implications for the mechanism of action of erythromycin.
    Biochimie. 1987 Aug;69(8):891-900 PMID: 2447958
  7. Susceptibility of Campylobacter pylori to macrolides and fluoroquinolones.
    J Antimicrob Chemother. 1988 Nov;22(5):631-6 PMID: 3209524
  8. Point mutations in the 23 S rRNA genes of four lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation.
    Mol Gen Genet. 1988 Oct;214(2):295-9 PMID: 3070353
  9. Effect of treatment of Helicobacter pylori infection on the long-term recurrence of gastric or duodenal ulcer. A randomized, controlled study.
    Ann Intern Med. 1992 May 1;116(9):705-8 PMID: 1558340
  10. Healing gastric MALT lymphomas by eradicating H pylori?
    Lancet. 1993 Sep 4;342(8871):568 PMID: 8102714
  11. Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori.
    Lancet. 1993 Sep 4;342(8871):575-7 PMID: 8102719
  12. Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTC11638.
    Mol Microbiol. 1994 Feb;11(3):509-23 PMID: 8152375
  13. Identification of mutations in 23S rRNA gene of clarithromycin-resistant Mycobacterium intracellulare.
    Antimicrob Agents Chemother. 1994 Feb;38(2):381-4 PMID: 8192472
  14. Helicobacter pylori.
    Am J Gastroenterol. 1994 Aug;89(8 Suppl):S116-28 PMID: 8048402
  15. Highly significant change of the clinical course of relapsing and complicated peptic ulcer disease after cure of Helicobacter pylori infection.
    Am J Gastroenterol. 1994 Oct;89(10):1785-8 PMID: 7942667
  16. Tight binding of clarithromycin, its 14-(R)-hydroxy metabolite, and erythromycin to Helicobacter pylori ribosomes.
    Antimicrob Agents Chemother. 1994 Jul;38(7):1496-500 PMID: 7979278
  17. Antibacterial treatment of gastric ulcers associated with Helicobacter pylori.
    N Engl J Med. 1995 Jan 19;332(3):139-42 PMID: 7800005
  18. Regression of primary gastric lymphoma of mucosa-associated lymphoid tissue type after cure of Helicobacter pylori infection. MALT Lymphoma Study Group.
    Lancet. 1995 Jun 24;345(8965):1591-4 PMID: 7783535
  19. Erythromycin resistance by ribosome modification.
    Antimicrob Agents Chemother. 1995 Mar;39(3):577-85 PMID: 7793855
  20. Site of action of a ribosomal RNA methylase responsible for resistance to erythromycin and other antibiotics.
    J Biol Chem. 1983 Oct 25;258(20):12702-6 PMID: 6195156
  21. Cluster analysis of Helicobacter pylori genomic DNA fingerprints suggests gastroduodenal disease-specific associations.
    Scand J Gastroenterol. 1995 Jul;30(7):640-6 PMID: 7481525
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.40.2.477
Email
[email protected]
Published
1996-02-00
Pages
477-80
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC163139
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.40.2.477 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2