Home LiteratureArticle Details
PMID: 8856342 Published · ppublish English Journal Article

The interaction of human fetal neurons and epidermal cells in vitro.

In vitro cellular & developmental biology. Animal ·Vol. 32 ·No. 7 ·1996-00-00 ·Pages 420-6

Penfold ME, Armati PJ, Mikloska Z, Cunningham AL

Abstract

The interaction of autologous human fetal neurons with epidermal cells was studied by culturing fetal dorsal root ganglia (DRG) in the center of a dual chamber system with epidermal explants in the outer chamber. The two chambers were separated by two concentric stainless steel annular rings adherent to the substratum by silicon grease and agarose. Axons from the DRG penetrated the agarose barrier, growing into the exterior chamber by 10 d in vitro (DIV) and extended past sparse peripheral fibroblasts to interact specifically with epidermal cells by 12 to 16 DIV. Scanning electron microscopy (SEM) showed single or multiple neuronal fascicles terminating on epidermal cells with spatular, veillike or bulbous axon termini. Transmission electron microscopy (TEM) showed fine axonal termini between epidermal cells, separated by an intercellular gap. The specificity of axonal targeting for epidermal cells rather than fibroblasts was also demonstrated by infecting the DRG with Herpes simplex virus type-1 (HSV-1). Specific anterograde transport of HSV-1 along axons to keratin-expressing epidermal cells was demonstrated by immunofluorescence and immunoperoxidase staining using monoclonal antibodies to viral glycoprotein D. This model allows the study of the mechanism of the specific interactions between neurons and epidermal cells analogous to those in fetal development and after cutaneous nerve regeneration.

MeSH Terms
Axons Cell Division Cells, Cultured Epidermal Cells Epidermis/physiology Ganglia, Spinal/cytology,embryology,physiology Humans Neurons/physiology
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Penfold M E
Virology Department, University of Sydney, ICPMR, Westmead Hospital, Sydney, Australia.
Armati P J
Mikloska Z
Cunningham A L
References (37)
37 references, click to expand
  1. Merkel cells in vitro: production of nerve growth factor and selective interactions with sensory neurons.
    Dev Biol. 1991 Apr;144(2):281-300 PMID: 2010033
  2. Neuritic transport of herpes simplex virus in rat sensory neurons in vitro. Effects of substances interacting with microtubular function and axonal flow [nocodazole, taxol and erythro-9-3-(2-hydroxynonyl)adenine].
    J Gen Virol. 1986 Sep;67 ( Pt 9):2023-8 PMID: 2427647
  3. Local control of neurite development by nerve growth factor.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4516-9 PMID: 270699
  4. Axon guidance by molecular gradients.
    Curr Opin Neurobiol. 1992 Feb;2(1):60-5 PMID: 1638137
  5. Cutaneous innervation in man visualized with protein gene product 9.5 (PGP 9.5) antibodies.
    Histochemistry. 1989;92 (5):385-90 PMID: 2531128
  6. Neurobiology. Just a chemical attraction.
    Nature. 1994 Mar 10;368(6467):101-2 PMID: 8139651
  7. A non-collagenous glycoprotein from elastic tissue acts as substratum for growth of cells in vitro.
    Nature. 1982 Feb 18;295(5850):614-5 PMID: 7057920
  8. Functional innervation of cultured skin grafts.
    J Invest Dermatol. 1992 Aug;99(2):120-8 PMID: 1629624
  9. Pathogenesis of herpes simplex virus infection and animal models for its study.
    Curr Top Microbiol Immunol. 1992;179:15-30 PMID: 1499348
  10. Growth-regulated synthesis and secretion of biologically active nerve growth factor by human keratinocytes.
    J Biol Chem. 1991 Nov 15;266(32):21718-22 PMID: 1718982
  11. Effects of tunicamycin on the avoidance reaction of epidermis by sensory neurites in co-cultures.
    Int J Dev Neurosci. 1990;8(3):245-54 PMID: 2386076
  12. Characterization of a factor that promotes neurite outgrowth: evidence linking activity to a heparan sulfate proteoglycan.
    J Cell Biol. 1982 Sep;94(3):574-85 PMID: 6215411
  13. Axon guidance and the patterning of neuronal projections in vertebrates.
    Science. 1988 Nov 4;242(4879):692-9 PMID: 3055291
  14. Restricted-diffusion chromatography through calibrated columns of granulated agar gel; a simple method for particle-size determination.
    Nature. 1962 Nov 3;196:475-6 PMID: 13983588
  15. Postnatal development of the cat's retina.
    Adv Anat Embryol Cell Biol. 1978;54(4):1-66 PMID: 356534
  16. Evidence that contact with connective tissue matrix is required for normal interaction between Schwann cells and nerve fibers.
    J Cell Biol. 1978 Sep;78(3):943-50 PMID: 701366
  17. Pathogenesis of herpetic neuritis and ganglionitis in mice: evidence for intra-axonal transport of infection.
    Infect Immun. 1973 Feb;7(2):272-88 PMID: 4348966
  18. Peripheral infection in culture of rat sensory neurons by herpes simplex virus.
    Infect Immun. 1980 May;28(2):620-3 PMID: 6249745
  19. Developmental mechanisms that generate precise patterns of neuronal connectivity.
    Cell. 1993 Jan;72 Suppl:77-98 PMID: 8428376
  20. Fetal skin development in vitro.
    In Vitro Cell Dev Biol. 1992 Apr;28A(4):223-6 PMID: 1582999
  21. Nerve fiber growth on defined hydrogel substrates.
    Science. 1982 May 21;216(4548):897-9 PMID: 7079743
  22. Axonal transport of herpes simplex virions to epidermal cells: evidence for a specialized mode of virus transport and assembly.
    Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6529-33 PMID: 7517552
  23. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
    J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
  24. Human Merkel cell regeneration in skin derived from cultured keratinocyte grafts.
    Lab Invest. 1990 Aug;63(2):233-41 PMID: 1696332
  25. Uptake and transport of herpes simplex virus in neurites of rat dorsal root ganglia cells in culture.
    J Gen Virol. 1984 Jan;65 ( Pt 1):55-64 PMID: 6319574
  26. UNC-6, a laminin-related protein, guides cell and pioneer axon migrations in C. elegans.
    Neuron. 1992 Nov;9(5):873-81 PMID: 1329863
  27. Intraepidermal nerves in human digital skin.
    Cell Tissue Res. 1988 Oct;254(1):111-7 PMID: 3197076
  28. A new medium for in vitro peripheral nervous tissue myelination without the use of antimitotics.
    J Neurosci Methods. 1990 Aug;33(2-3):149-55 PMID: 2232866
  29. Insulin, insulin-like growth factor II, and nerve growth factor effects on tubulin mRNA levels and neurite formation.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):7126-30 PMID: 3901011
  30. Morphological and biochemical maturation of neurones cultured in the absence of glial cells.
    Nature. 1979 Oct 4;281(5730):378-80 PMID: 39256
  31. Intraepidermal formation of Merkel cells in xenografts of human fetal skin.
    J Invest Dermatol. 1990 Mar;94(3):359-64 PMID: 2407788
  32. Structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2.
    J Virol. 1980 May;34(2):521-31 PMID: 6154808
  33. Neuronal cell-cell adhesion depends on interactions of N-CAM with heparin-like molecules.
    Nature. 1986 Apr 3-9;320(6061):445-7 PMID: 3083264
  34. In vitro analysis of interactions between sensory neurons and skin: evidence for selective innervation of dermis and epidermis.
    J Embryol Exp Morphol. 1985 Apr;86:53-70 PMID: 4031747
  35. Turning of nerve growth cones induced by neurotransmitters.
    Nature. 1994 Mar 10;368(6467):140-4 PMID: 8139655
  36. Comparative biology of latent varicella-zoster virus and herpes simplex virus infections.
    J Infect Dis. 1992 Aug;166 Suppl 1:S13-23 PMID: 1320646
  37. Purification and separation of tobacco mosaic virus and southern bean mosaic virus by agar-gel filtration.
    Nature. 1962 Apr 7;194:114-6 PMID: 13916507
Article Info
Journal
In vitro cellular & developmental biology. Animal
Abbr.
In Vitro Cell Dev Biol Anim
ISSN
1071-2690
DOI
10.1007/BF02723004
Published
1996-00-00
Pages
420-6
Language
English
Region
Germany
NLM ID
9418515
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02723004 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2