Abstract
The role of the newly identified p38 mitogen-activated protein kinase (MAP kinase) in terminally differentiated cells, such as human neutrophils, is totally unknown. In order to examine the possible role of this MAP kinase in the phosphorylation and activation of cytoplasmic phospholipase A2 (cPLA2), we tested the effect of the recently synthesized inhibitor of p38 MAP kinase, SB 203580, on the phosphorylation and activation of both p38 MAP kinase and cPLA2. We found that while tumour necrosis factor-alpha (TNF-alpha)-stimulated tyrosine phosphorylation of p38 MAP kinase is affected only slightly by SB 203580, its stimulated kinase activity is greatly reduced in human neutrophils in suspension treated with this inhibitor. Furthermore, the TNF-alpha-stimulated phosphorylation and activation of cPLA2 are completely abolished in cells treated with SB 203580. Based on these data, it is reasonable to conclude that an SB 203580-sensitive kinase, or kinases and/or phosphatases, are involved in the phosphorylation and activation of cPLA2 in intact human neutrophils in suspension stimulated by TNF-alpha. The possible role of the p38 MAP kinase cascade in the phosphorylation and activation of cPLA2 is discussed.
MeSH Terms
Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors,blood
Cytosol/enzymology
Enzyme Activation
Enzyme Inhibitors/pharmacology
Humans
Imidazoles/pharmacology
JNK Mitogen-Activated Protein Kinases
MAP Kinase Kinase 4
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Neutrophils/drug effects,metabolism
Phospholipases A/blood
Phospholipases A2
Phosphorylation
Protein Kinases/blood
Pyridines/pharmacology
Tumor Necrosis Factor-alpha/pharmacology
p38 Mitogen-Activated Protein Kinases
Chemicals
Enzyme Inhibitors
Imidazoles
Pyridines
Tumor Necrosis Factor-alpha
Protein Kinases
Calcium-Calmodulin-Dependent Protein Kinases
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases
p38 Mitogen-Activated Protein Kinases
MAP Kinase Kinase 4
Mitogen-Activated Protein Kinase Kinases
Phospholipases A
Phospholipases A2
SB 203580
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Waterman W H
Department of Physiology, University of Connecticut Health Center, Farmington 06030, USA.
Molski T F
Huang C K
Adams J L
Sha'afi R I
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