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PMID: 887939 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A specific high-affinity binding macromolecule for 1,25-dihydroxyvitamin D3 in fetal bone.

Science (New York, N.Y.) ·Vol. 197 ·No. 4308 ·1977-09-09 ·Pages 1086-8

Kream BE, Jose M, Yamada S, DeLuca HF

Abstract

Cytosol fractions were prepared from fetal rat or embryonic chick calvaria and analyzed for binding of vitamin D3 metabolites on sucrose density gradients. Both cytosol fractions contain a 3.5S macromolecule which specifically binds 1,25-dihydroxy-[3H]vitamin D3 and in addition, a 5 to 6S macromolecule which binds 25-hydroxy-[3H]vitamin D3. In rat calvaria cytosol, 1,25-dihydroxy-[3H]vitamin D3 also binds to the 5 to 6S macromolecule but appears to have greater affinity for the 3.5S component.

MeSH Terms

Animals Bone and Bones/embryology,metabolism Carrier Proteins/metabolism Chick Embryo Cytosol/metabolism Dihydroxycholecalciferols/metabolism Hydroxycholecalciferols/metabolism In Vitro Techniques Rats Receptors, Steroid

Chemicals

Carrier Proteins Dihydroxycholecalciferols Hydroxycholecalciferols Receptors, Steroid

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kream B E

Jose M

Yamada S

DeLuca H F

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.887939

Published

1977-09-09

Pages

1086-8

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.887939 View journal details

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A specific high-affinity binding macromolecule for 1,25-dihydroxyvitamin D3 in fetal bone.

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