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PMID: 8887551 Published · ppublish English Journal Article

Spc110p: assembly properties and role in the connection of nuclear microtubules to the yeast spindle pole body.

The EMBO journal ·Vol. 15 ·No. 17 ·1996-09-02 ·Pages 4592-602

Kilmartin JV, Goh PY

Abstract

Spc110p is an essential component of the budding yeast spindle pole body (SPB). It binds calmodulin and contains a long central coiled-coil rod which acts as a spacer element between the central plaque of the SPB and the ends of the nuclear or spindle microtubules. This suggests that the essential function of Spc110p is to connect the nuclear microtubules to the SPB. To confirm this, we examined the phenotype of ts alleles of SPC110, one of which contains a mutation in the calmodulin binding site and was suppressed by overexpression of calmodulin. The alleles fail to form a functional mitotic spindle because spindle microtubules are not properly connected to the SPB. We also examined the phenotype of the toxic overexpression of either the wild-type or a truncated version of Spc110p containing a deletion of most of the coiled-coil domain. Both of these proteins form large ordered spheroidal polymers in the nucleus. The polymerization of the truncated Spc110p appears to be initiated inside the SPB from the position where Spc110p is normally located, and as the polymer grows in size it severs the connection between the nuclear microtubules and the SPB. The polymers were purified and are composed of Spc110p and calmodulin. A model for the structure of the polymer is proposed.

MeSH Terms
Binding Sites Calmodulin/metabolism Calmodulin-Binding Proteins Cell Nucleus/metabolism Cytoskeletal Proteins Fungal Proteins/metabolism Microscopy, Immunoelectron Microtubules/metabolism Mutation Nuclear Proteins/metabolism Phenotype Saccharomyces cerevisiae/metabolism,ultrastructure Saccharomyces cerevisiae Proteins Spindle Apparatus
Chemicals
Calmodulin Calmodulin-Binding Proteins Cytoskeletal Proteins Fungal Proteins Nuclear Proteins SPC110 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kilmartin J V
MRC Laboratory of Molecular Biology, Cambridge, UK.
Goh P Y
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-09-02
Pages
4592-602
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452189
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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