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PMID: 8887639 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A novel DNA binding motif for yeast zinc cluster proteins: the Leu3p and Pdr3p transcriptional activators recognize everted repeats.

Molecular and cellular biology ·Vol. 16 ·No. 11 ·1996-11-00 ·Pages 6096-102

Hellauer K, Rochon MH, Turcotte B

Abstract

The Gal4, Put3, and Ppr1 yeast zinc cluster proteins bind as homodimers to DNA sequences composed of palindromic CGG triplets. Spacing between the triplets specifies the target site for a given zinc cluster protein. In addition, Hap1p, another zinc cluster protein, also recognizes CGG triplets but only when oriented as a direct repeat. Unexpectedly, our results show that Leu3p, another member of this family, also recognizes CGG triplets but oriented in opposite directions and spaced by 4 nucleotides (an everted repeat or inverted palindrome: CCG-N4-CGG). This constitutes a novel DNA motif for zinc cluster proteins. Moreover, the presence of this motif was shown to be essential for in vivo activation by Leu3p of a minimal reporter containing one copy of a target site for this activator. We also provide evidence that another member of this family, Pdr3p, binds to an everted repeat spaced by 0 nucleotides (CCGCGG). Thus, our results show that three CGG motifs are used by members of the zinc cluster family: palindromes, direct repeats, and everted repeats.

MeSH Terms
Base Sequence Binding Sites DNA/chemistry,metabolism DNA Primers DNA-Binding Proteins/metabolism Dimerization Fungal Proteins/metabolism Genes, Reporter Nuclear Proteins/metabolism Polymerase Chain Reaction Recombinant Proteins/biosynthesis Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Trans-Activators/metabolism Transcription Factors/metabolism Trinucleotide Repeats Zinc Fingers beta-Galactosidase/biosynthesis
Chemicals
DNA Primers DNA-Binding Proteins Fungal Proteins LEU3 protein, S cerevisiae Nuclear Proteins PDR3 protein, S cerevisiae Recombinant Proteins Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors DNA beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hellauer K
Department of Medicine, Royal Victoria Hospital, Québec, Canada.
Rochon M H
Turcotte B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.11.6096
Published
1996-11-00
Pages
6096-102
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231612
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.11.6096 PMC Full Text View journal details
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