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PMID: 8893021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of synapse structure and function by the Drosophila tumor suppressor gene dlg.

Neuron ·Vol. 17 ·No. 4 ·1996-10-00 ·Pages 627-40

Budnik V, Koh YH, Guan B, Hartmann B, Hough C, Woods D, Gorczyca M

Abstract

Mutations of the tumor suppressor gene discs-large (dlg) lead to postsynaptic structural defects. Here, we report that mutations in dlg also result in larger synaptic currents at fly neuromuscular junctions. By selectively targeting DLG protein to either muscles or motorneurons using Gal-4 enhancer trap lines, we were able to rescue substantially the reduced postsynaptic structure in mutants. Rescue of the physiological defect was accomplished by presynaptic, but not postsynaptic targeting, consistent with our finding that miniature excitatory junctional currents were not changed in dlg mutants. These results suggest that DLG functions in the regulation of neurotransmitter release and postsynaptic structure. We propose that DLG is an integral part of a mechanism by which changes in both neurotransmitter release and synapse structure are accomplished during development and plasticity.

MeSH Terms
Animals Axons Drosophila/genetics,physiology Drosophila Proteins Evoked Potentials Gene Expression Regulation, Developmental Genes, Insect Genes, Tumor Suppressor Insect Hormones/biosynthesis,genetics Microscopy, Electron Motor Neurons/physiology,ultrastructure Muscles/innervation Mutagenesis Neuromuscular Junction/physiology,ultrastructure Synapses/physiology,ultrastructure Synaptic Transmission Tumor Suppressor Proteins
Chemicals
Drosophila Proteins Insect Hormones Tumor Suppressor Proteins dlg1 protein, Drosophila
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Budnik V
Department of Biology, University of Massachusetts, Amherst 01003, USA.
Koh Y H
Guan B
Hartmann B
Hough C
Woods D
Gorczyca M
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/s0896-6273(00)80196-8
Published
1996-10-00
Pages
627-40
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC4661176
Subset
IM
Grants
NINDS NIH HHS · R01 NS030072 · United States
NINDS NIH HHS · R21 NS070032 · United States
NINDS NIH HHS · K04 NS01786 · United States
NINDS NIH HHS · R01 NS70032 · United States
External Links
PubMed source DOI: 10.1016/s0896-6273(00)80196-8 PMC Full Text View journal details
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