Lipoxygenase activity in heart cells.

FEBS letters ·Vol. 395 ·No. 2-3 ·1996-10-21 ·Pages 148-52

Breitbart E, Sofer Y, Shainberg A, Grossman S

Abstract

Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[14C]AA. LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.

MeSH Terms

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid/isolation & purification,metabolism Animals Arachidonic Acid/metabolism Cells, Cultured Cytosol/enzymology Female Hydroxyeicosatetraenoic Acids/isolation & purification,metabolism Kinetics Lipoxygenase/metabolism Microsomes/enzymology Myocardium/enzymology Rats

Chemicals

Hydroxyeicosatetraenoic Acids Arachidonic Acid 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid 15-hydroxy-5,8,11,13-eicosatetraenoic acid Lipoxygenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Breitbart E

Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.

Sofer Y

Shainberg A

Grossman S

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(96)01017-4

Published

1996-10-21

Pages

148-52

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(96)01017-4 View journal details

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Lipoxygenase activity in heart cells.

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