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PMID: 8913755 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of dominant enhancers and suppressors of activated Notch in Drosophila.

Genetics ·Vol. 144 ·No. 3 ·1996-11-00 ·Pages 1127-41

Verheyen EM, Purcell KJ, Fortini ME, Artavanis-Tsakonas S

Abstract

The Notch receptor controls cell fate decisions throughout Drosophila development. Truncated, ligand-independent forms of this protein delay or block differentiation. We have previously shown that expression of the intracellular domain of the receptor under the control of the sevenless enhancer/promoter induces a rough eye phenotype in the adult fly. Analysis of the resultant cellular transformations suggested that this form of Notch acts as a constitutively activated receptor. To identify gene products that interact with Notch, a second-site mutagenesis screen was performed to isolate enhancers and suppressors of the eye phenotype caused by expression of these activated Notch molecules. We screened 137,000 mutagenized flies and recovered 290 dominant modifiers. Many new alleles of previously identified genes were isolated, as were mutations defining novel loci that may function in the Notch signaling pathway. We discuss the data with respect to known features of Notch receptor signaling and Drosophila eye development.

MeSH Terms
Animals Drosophila/genetics Drosophila Proteins Enhancer Elements, Genetic Eye/ultrastructure Female Genes, Suppressor Genetic Complementation Test Male Membrane Proteins/genetics Mutagenesis, Site-Directed Receptors, Cell Surface/genetics Receptors, Notch Signal Transduction/genetics
Chemicals
Drosophila Proteins Membrane Proteins N protein, Drosophila Receptors, Cell Surface Receptors, Notch
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Verheyen E M
Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06536, USA.
Purcell K J
Fortini M E
Artavanis-Tsakonas S
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/144.3.1127
Published
1996-11-00
Pages
1127-41
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207606
Subset
IM
Grants
NINDS NIH HHS · NS-26084 · United States
External Links
PubMed source DOI: 10.1093/genetics/144.3.1127 PMC Full Text View journal details
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