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PMID: 8917110 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Alternate splice-site utilization in the gene for the catalytic subunit of the DNA-activated protein kinase, DNA-PKcs.

Gene ·Vol. 175 ·No. 1-2 ·1996-10-10 ·Pages 271-3

Connelly MA, Zhang H, Kieleczawa J, Anderson CW

Abstract

Analysis of polyA-selected RNAs and cDNA clones from several human cell lines revealed the presence of a 93-bp exon in the PI kinase homology region of DNA-PKcs that was not present in the cDNA sequence derived from HeLa cell cDNA clones. RT-PCR showed that most DNA-PKcs mRNAs in human cells have this exon. Thus, the nascent DNA-PKcs polypeptide is composed of 4127 aa and has a predicted mol. wt. of 469021 (470 kDa).

MeSH Terms

Alternative Splicing/genetics Amino Acid Sequence Base Sequence DNA-Activated Protein Kinase DNA-Binding Proteins Exons/genetics HeLa Cells Humans Molecular Sequence Data Nuclear Proteins Open Reading Frames/genetics Protein Serine-Threonine Kinases/genetics RNA, Messenger/genetics

Chemicals

DNA-Binding Proteins Nuclear Proteins RNA, Messenger DNA-Activated Protein Kinase PRKDC protein, human Protein Serine-Threonine Kinases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Connelly M A

Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA.

Zhang H

Kieleczawa J

Anderson C W

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(96)00135-7

Published

1996-10-10

Pages

271-3

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Databases

GENBANK

U34994, U47077

External Links

PubMed source DOI: 10.1016/0378-1119(96)00135-7 View journal details

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Alternate splice-site utilization in the gene for the catalytic subunit of the DNA-activated protein kinase, DNA-PKcs.

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