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PMID: 8917596 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heteromultimeric CLC chloride channels with novel properties.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 23 ·1996-11-12 ·Pages 13362-6

Lorenz C, Pusch M, Jentsch TJ

Abstract

The skeletal muscle chloride channel CLC-1 and the ubiquitous volume-activated chloride channel CLC-2 belong to a large gene family whose members often show overlapping expression patterns. CLC-1 and CLC-2 are coexpressed in skeletal and smooth muscle and in the heart. By coexpressing CLC-1 and CLC-2 in Xenopus oocytes, we now show the formation of novel CLC-1/CLC-2 heterooligomers that yield time-independent linear chloride currents with a chloride-->bromide-->iodide selectivity sequence. Formation of heterooligomeric CLC channels increases the number and possible functions of chloride channels.

MeSH Terms
Animals Anthracenes/pharmacology CLC-2 Chloride Channels Cell Membrane/drug effects,physiology Chloride Channels/biosynthesis,chemistry,physiology Female Macromolecular Substances Membrane Potentials/drug effects Multigene Family Muscle Proteins/biosynthesis,chemistry,physiology Muscle, Skeletal/metabolism Mutagenesis, Site-Directed Nerve Tissue Proteins/biosynthesis,chemistry,physiology Oocytes/physiology Patch-Clamp Techniques Point Mutation Protein Multimerization RNA, Complementary Xenopus laevis
Chemicals
Anthracenes CLC-1 channel CLC-2 Chloride Channels Chloride Channels Macromolecular Substances Muscle Proteins Nerve Tissue Proteins RNA, Complementary 9-anthroic acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lorenz C
Center for Molecular Neurobiology, Hamburg University, Germany.
Pusch M
Jentsch T J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.23.13362
Published
1996-11-12
Pages
13362-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24098
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.23.13362 PMC Full Text View journal details
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