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PMID: 8920874 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Schistosoma mansoni egg-induced early IL-4 production is dependent upon IL-5 and eosinophils.

The Journal of experimental medicine ·Vol. 184 ·No. 5 ·1996-11-01 ·Pages 1871-8

Sabin EA, Kopf MA, Pearce EJ

Abstract

The initial immune response to Schistosoma mansoni eggs presumably results in IL-4 production, as schistosome eggs are strong Th2-inducing antigens and the differentiation of antigen-specific Th2 cells is largely dependent on the presence of IL-4 during priming of naive Th cells. Consistent with this concept, intraperitoneal injection of mice with schistosome eggs results in an upregulation of IL-4 production by peritoneal exudate cells (PECs) within 12 h. Egg-induced IL-4 is rapidly bound by its receptor, suggesting that this cytokine is utilized by a cell type present at the site of antigen deposition or is complexed to soluble receptor. The peak of early IL-4 production is accompanied by a local eosinophilia and the apparent disappearance of mast cells. Studies utilizing either IL-4, IL-5, or mast cell-deficient mice indicate that the eosinophilia is dependent on mast cells and IL-5 and independent of IL-4. Strikingly, egg-induced IL-4 production is absent in animals lacking the early peritoneal eosinophilia. Immunocytochemical analysis of PEC following egg injection indicates that the eosinophils themselves make IL-4. These data strongly suggest that egg-induced IL-5 plays an essential role in recruiting eosinophils to the site of antigen deposition and that it is these eosinophils that then directly produce early IL-4.

MeSH Terms
Animals Antigens, CD/metabolism Eosinophilia Eosinophils/immunology,metabolism Interleukin-4/metabolism Interleukin-5/metabolism Mast Cells/immunology Mice Models, Immunological Ovum/immunology Peritoneal Cavity/cytology Receptors, Interleukin/metabolism Receptors, Interleukin-4 Schistosoma mansoni/immunology Time Factors Up-Regulation
Chemicals
Antigens, CD Interleukin-5 Receptors, Interleukin Receptors, Interleukin-4 Interleukin-4
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sabin E A
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA.
Kopf M A
Pearce E J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.5.1871
Published
1996-11-01
Pages
1871-8
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192874
Subset
IM
Grants
NIAID NIH HHS · AI09227 · United States
NIAID NIH HHS · AI32573 · United States
External Links
PubMed source DOI: 10.1084/jem.184.5.1871 PMC Full Text View journal details
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