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PMID: 8926044 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Candida albicans induces the release of inflammatory mediators from human peripheral blood monocytes.

Inflammation ·Vol. 20 ·No. 1 ·1996-02-00 ·Pages 107-22

Castro M, Bjoraker JA, Rohrbach MS, Limper AH

Abstract

Candida albicans (C. albicans) is a major nosocomial pathogen. We examined arachidonic acid (AA) and cytokine production by monocytes stimulated with C. albicans. [14C]-AA labeled monocytes released 8.9 +/- 2.3% of the incorporated AA following stimulation with live C. albicans (C. albicans: monocyte of 16:1) (P = 0.0002). Prior studies indicate that soluble alpha-mannans and beta-glucans antagonize mannose and beta-glucan receptors, respectively. Preincubation of monocytes with alpha-mannan (100 micrograms/ml) caused 45.8 +/- 5.7% inhibition of [14C]-AA release, whereas beta-glucan (100 micrograms/ml) yielded 43.7 +/- 6.0% inhibition (P < 0.05 for each compared to control). Additionally, monocytes stimulated with C. albicans also released interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF alpha), interleukin-6 (IL-6) and interleukin-8 (IL-8). However, alpha-mannan or beta-glucan failed to inhibit IL-1 beta release. These data indicate that C. albicans induces monocytes to release AA and inflammatory cytokines. Furthermore, AA, but not cytokine liberation, is partially mediated by alpha-mannan and beta-glucan components of the fungus.

MeSH Terms
Arachidonic Acid/metabolism Candida albicans/chemistry,physiology Cell Wall/chemistry Cells, Cultured Cytokines/biosynthesis,genetics,metabolism Gene Expression Regulation/drug effects Glucans/pharmacology Humans Interleukin-1/metabolism Interleukin-6/metabolism Interleukin-8/metabolism Lectins, C-Type Mannans/pharmacology Mannose Receptor Mannose-Binding Lectins Monocytes/drug effects,metabolism Oligosaccharides/pharmacology Receptors, Cell Surface/drug effects,physiology Receptors, Immunologic/drug effects,physiology Tumor Necrosis Factor-alpha/metabolism
Chemicals
Cytokines Glucans Interleukin-1 Interleukin-6 Interleukin-8 Lectins, C-Type Mannans Mannose Receptor Mannose-Binding Lectins Oligosaccharides Receptors, Cell Surface Receptors, Immunologic Tumor Necrosis Factor-alpha beta-glucan receptor laminariheptaose Arachidonic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Castro M
Division of Pulmonary and Critical Care, Mayo Clinic, Rochester, MN, USA.
Bjoraker J A
Rohrbach M S
Limper A H
References (20)
20 references, click to expand
  1. Mechanisms of host defense against Candida species. I. Phagocytosis by monocytes and monocyte-derived macrophages.
    J Immunol. 1991 Apr 15;146(8):2783-9 PMID: 1901885
  2. Candida mannan: chemistry, suppression of cell-mediated immunity, and possible mechanisms of action.
    Clin Microbiol Rev. 1991 Jan;4(1):1-19 PMID: 2004345
  3. Pathogenesis of candidiasis. Immunosuppression by cell wall mannan catabolites.
    Arch Surg. 1989 Nov;124(11):1290-4 PMID: 2684094
  4. A beta-glucan inhibitable receptor on human monocytes: its identity with the phagocytic receptor for particulate activators of the alternative complement pathway.
    J Immunol. 1985 Apr;134(4):2588-93 PMID: 2579146
  5. Separation of immunomodulatory effects of mannan from Candida albicans into stimulatory and suppressive components.
    Cell Immunol. 1986 Sep;101(2):403-14 PMID: 3530504
  6. Different effects of native Candida albicans mannan and mannan-derived oligosaccharides on antigen-stimulated lymphoproliferation in vitro.
    J Immunol. 1990 Jan 15;144(2):707-16 PMID: 2153174
  7. Uptake of Pneumocystis carinii mediated by the macrophage mannose receptor.
    Nature. 1991 May 9;351(6322):155-8 PMID: 1903183
  8. Pneumocystis carinii stimulates tumor necrosis factor-alpha release from alveolar macrophages through a beta-glucan-mediated mechanism.
    J Immunol. 1993 May 1;150(9):3932-40 PMID: 8386203
  9. Phagocytosis and intracellular killing of pathogenic yeasts by human monocytes and neutrophils.
    Infect Immun. 1979 Jun;24(3):932-8 PMID: 381207
  10. Generation of leukotrienes by human monocytes upon stimulation of their beta-glucan receptor during phagocytosis.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2751-5 PMID: 2986134
  11. Isolation of soluble yeast beta-glucans that inhibit human monocyte phagocytosis mediated by beta-glucan receptors.
    J Immunol. 1986 Nov 15;137(10):3270-6 PMID: 3021849
  12. The effect of a mannose binding protein on macrophage interactions with Candida albicans.
    Cell Mol Biol. 1992 Jul;38(4):407-12 PMID: 1499040
  13. Zymosan induces selective release of arachidonic acid from rabbit alveolar macrophages via stimulation of a beta-glucan receptor.
    FEBS Lett. 1992 Sep 7;309(2):119-22 PMID: 1324190
  14. Evidence that mannans of Candida albicans are responsible for adherence of yeast forms to spleen and lymph node tissue.
    Infect Immun. 1993 Jun;61(6):2578-84 PMID: 8500895
  15. CELL WALL OF YEASTS.
    Annu Rev Microbiol. 1963;17:15-30 PMID: 14145300
  16. Candida albicans stimulates endothelial cell eicosanoid production.
    J Infect Dis. 1991 Nov;164(5):928-35 PMID: 1940471
  17. The cell wall of fungal human pathogens: its possible role in host-parasite relationships.
    Mycopathologia. 1982 Sep 17;79(3):159-84 PMID: 6755258
  18. Risk factors for hospital-acquired candidemia. A matched case-control study.
    Arch Intern Med. 1989 Oct;149(10):2349-53 PMID: 2802900
  19. Candida albicans stimulates arachidonic acid liberation from alveolar macrophages through alpha-mannan and beta-glucan cell wall components.
    Infect Immun. 1994 Aug;62(8):3138-45 PMID: 8039882
  20. Isolation and structure of glucan from regenerating spheroplasts of Candida albicans.
    J Gen Microbiol. 1984 May;130(5):1217-25 PMID: 6381644
Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1007/BF01487749
Published
1996-02-00
Pages
107-22
Language
English
Region
United States
NLM ID
7600105
Subset
IM
Grants
NIAID NIH HHS · AI 34336 · United States
NHLBI NIH HHS · HL-28669 · United States
External Links
PubMed source DOI: 10.1007/BF01487749 View journal details
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