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PMID: 8927511 Published · ppublish English Journal Article

The role of sarcoplasmic reticulum and sarcoplasmic reticulum Ca2+-ATPase in the smooth muscle tone of the cat gastric fundus.

Pflugers Archiv : European journal of physiology ·Vol. 431 ·No. 6 ·1996-04-00 ·Pages 928-35

Petkov GV, Boev KK

Abstract

Circular smooth muscle strips isolated from cat gastric fundus were studied in order to understand whether the sarcoplasmic reticulum (SR) and SR Ca2+-ATPase could play a role in the regulation of the muscle tone. Cyclopiazonic acid (CPA), a specific inhibitor of SR Ca2+-ATPase, caused a significant and sustained increase in muscle tone, depending on the presence of extracellular Ca2+. Nifedipine and cinnarizin only partially suppressed the CPA-induced tonic contraction. Bay K 8644 antagonized the relaxant effect of nifedipine in CPA-contracted fundus. Nitric-oxide-releasing agents sodium nitroprusside and 3-morpholino-sydnonimine completely suppressed the CPA-induced tonic contraction. The blockers of Ca2+-activated K+ channels, tetraethylammonium, charybdotoxin and/or apamin, decreased the contractile effect of CPA. Vanadate increased the tone but did not change significantly the effect of CPA. CPA exerted its contractile effect even when Ca2+ influx was triggered through the Na+/Ca2+ exchanger and the other Ca2+ entry pathways were blocked. Thapsigargin, another specific SR Ca2+-ATPase inhibitor, also increased the muscle tone. The effect of thapsigargin was completely suppressed by sodium nitroprusside and 3-morpholino-sydnonimine and partially by nifedipine. In conclusion, under conditions when the SR Ca2+-ATPase is inhibited, the tissue develops a strong tonic contraction and a large part of this is mediated by Ca2+ influx presumably via nifedipine-sensitive Ca2+ channels. This study suggests the important role of SR Ca2+-ATPase in the modulation of the muscle tone and the function of SR as a "buffer barrier" to Ca2+ entry in the cat gastric fundus smooth muscle.

MeSH Terms
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology Animals Apamin/pharmacology Calcium Channel Agonists/pharmacology Calcium Channel Blockers/pharmacology Calcium-Transporting ATPases/antagonists & inhibitors,physiology Cats Cinnarizine/pharmacology Enzyme Inhibitors/pharmacology Gastric Fundus/drug effects,enzymology,physiology In Vitro Techniques Indoles/pharmacology Male Molsidomine/analogs & derivatives,pharmacology Muscle Contraction/drug effects,physiology Muscle, Smooth/drug effects,enzymology,physiology Nifedipine/pharmacology Nitroprusside/pharmacology Potassium Channel Blockers Sarcoplasmic Reticulum/drug effects,enzymology,physiology Thapsigargin/pharmacology
Chemicals
Calcium Channel Agonists Calcium Channel Blockers Enzyme Inhibitors Indoles Potassium Channel Blockers Nitroprusside Apamin Cinnarizine linsidomine Thapsigargin 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester Molsidomine Calcium-Transporting ATPases Nifedipine cyclopiazonic acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Petkov G V
Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street., Bl. 21, 1113-Sofia, Bulgaria.
Boev K K
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/s004240050087
Published
1996-04-00
Pages
928-35
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/s004240050087 View journal details
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