Abstract
We report the identification of a family of sequences located by in situ hybridisation to the centromeres of all the Triticeae chromosomes studied, including the supernumerary and midget chromosomes, the centromeres of all maize chromosomes and the heterochromatic regions of rice chromosomes. This family of sequences (CCS1), together with the cereal genome alignments, will allow the evolution of the cereal centromeres and their sites to be studied. The family of sequences also shows homology to the CENP-B box. The centromeres of the cereal species and the proteins that interact with them can now be characterised.
MeSH Terms
Autoantigens
Base Sequence
Binding Sites
Centromere/genetics
Centromere Protein B
Chromosomal Proteins, Non-Histone
DNA, Plant/genetics
DNA-Binding Proteins
Edible Grain/genetics
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid/genetics
Sequence Alignment
Sequence Homology, Nucleic Acid
Chemicals
Autoantigens
Centromere Protein B
Chromosomal Proteins, Non-Histone
DNA, Plant
DNA-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aragón-Alcaide L
Cereals Department, John Innes Centre, Colney lane, Norwich, NR4 7UH, UK.
Miller T
Schwarzacher T
Reader S
Moore G
References (24)
24 references, click to expand
-
Molecular and physical organization of highly repetitive, undermethylated DNA from Pennisetum glaucum.
Mol Gen Genet. 1994 Aug 15;244(4):420-5
PMID: 7521511
-
A family of related sequences associated with (TTTAGGG)n repeats are located in the interstitial regions of wheat chromosomes.
Mol Gen Genet. 1994 Nov 1;245(3):349-54
PMID: 7816045
-
Mouse minor satellite DNA genetically maps to the centromere and is physically linked to the proximal telomere.
Genomics. 1991 Oct;11(2):235-41
PMID: 1685135
-
Centromeric sites and cereal chromosome evolution.
Chromosoma. 1997 Apr;105(6):321-3
PMID: 9087373
-
De novo formation of several features of a centromere following introduction of a Y alphoid YAC into mammalian cells.
Hum Mol Genet. 1994 May;3(5):689-95
PMID: 8081354
-
Centromeric repetitive DNA sequences in the genus Brassica.
Theor Appl Genet. 1995 Feb;90(2):157-65
PMID: 24173886
-
Molecular characterization of a maize B chromosome centric sequence.
Genetics. 1993 Oct;135(2):589-97
PMID: 8244015
-
Repetitive DNA and chromosome evolution in plants.
Philos Trans R Soc Lond B Biol Sci. 1986 Jan 29;312(1154):227-42
PMID: 2870519
-
A simple method using T4 DNA polymerase to clone polymerase chain reaction products.
Biotechniques. 1994 Aug;17(2):236-8
PMID: 7980913
-
Mouse centromere mapping using oligonucleotide probes that detect variants of the minor satellite.
Chromosoma. 1994 Mar;103(1):46-55
PMID: 8013255
-
Genetic and physical mapping of telomeres and macrosatellites of rice.
Plant Mol Biol. 1993 Aug;22(5):861-72
PMID: 8102907
-
Comparative linkage maps of the rice and maize genomes.
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7980-4
PMID: 8103599
-
The centromere frontier: kinetochore components, microtubule-based motility, and the CEN-value paradox.
Cell. 1993 May 21;73(4):621-4
PMID: 8500159
-
Isolation of a higher eukaryotic telomere from Arabidopsis thaliana.
Cell. 1988 Apr 8;53(1):127-36
PMID: 3349525
-
Key features of cereal genome organization as revealed by the use of cytosine methylation-sensitive restriction endonucleases.
Genomics. 1993 Mar;15(3):472-82
PMID: 8468041
-
Centromeres of mammalian chromosomes.
Trends Genet. 1990 Dec;6(12):410-6
PMID: 2087784
-
Conservation of fine-scale DNA marker order in the genomes of rice and the Triticeae.
Nucleic Acids Res. 1995 Jul 25;23(14):2724-8
PMID: 7651833
-
Cereal genome evolution. Grasses, line up and form a circle.
Curr Biol. 1995 Jul 1;5(7):737-9
PMID: 7583118
-
Was there a single ancestral cereal chromosome?
Trends Genet. 1995 Mar;11(3):81-2
PMID: 7732576
-
CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins.
Chromosoma. 1991 Jul;100(6):360-70
PMID: 1893793
-
Organization of retro-element and stem-loop repeat families in the genomes and nuclei of cereals.
Chromosome Res. 1995 Jan;3(1):5-15
PMID: 7704416
-
BIS 1, a major component of the cereal genome and a tool for studying genomic organization.
Genomics. 1991 Jun;10(2):469-76
PMID: 2071151
-
Cereal genome evolution: pastoral pursuits with 'Lego' genomes.
Curr Opin Genet Dev. 1995 Dec;5(6):717-24
PMID: 8745069
-
Mouse satellite DNA, centromere structure, and sister chromatid pairing.
J Cell Biol. 1986 Oct;103(4):1145-51
PMID: 2429969