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PMID: 8941645 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of tachykinin gene and peptide expression in guinea pig nodose primary afferent neurons by allergic airway inflammation.

The Journal of clinical investigation ·Vol. 98 ·No. 10 ·1996-11-15 ·Pages 2284-91

Fischer A, McGregor GP, Saria A, Philippin B, Kummer W

Abstract

Substance P (SP), neurokinin A (NKA), and calcitonin gene-related peptide (CGRP) have potent proinflammatory effects in the airways. They are released from sensory nerve endings originating in jugular and dorsal root ganglia. However, the major sensory supply to the airways originates from the nodose ganglion. In this study, we evaluated changes in neuropeptide biosynthesis in the sensory airway innervation of ovalbumin-sensitized and -challenged guinea pigs at the mRNA and peptide level. In the airways, a three- to fourfold increase of SP, NKA, and CGRP, was seen 24 h following allergen challenge. Whereas no evidence of local tachykinin biosynthesis was found 12 h after challenge, increased levels of preprotachykinin (PPT)-A mRNA (encoding SP and NKA) were found in nodose ganglia. Quantitative in situ hybridization indicated that this increase could be accounted for by de novo induction of PPT-A mRNA in nodose ganglion neurons. Quantitative immunohistochemistry showed that 24 h after challenge, the number of tachykinin-immunoreactive nodose ganglion neurons had increased by 25%. Their projection to the airways was shown. Changes in other sensory ganglia innervating the airways were not evident. These findings suggest that an induction of sensory neuropeptides in nodose ganglion neurons is crucially involved in the increase of airway hyperreactivity in the late response to allergen challenge.

MeSH Terms
Animals Blotting, Northern Calcitonin Gene-Related Peptide/analysis,biosynthesis Female Gene Expression Regulation Guinea Pigs Immunohistochemistry In Situ Hybridization Inflammation/genetics Microscopy, Electron, Scanning Neurokinin A/analysis,biosynthesis Neurons, Afferent/immunology Nodose Ganglion/cytology Ovalbumin/immunology RNA, Messenger/analysis,biosynthesis Radioimmunoassay Respiratory Hypersensitivity/genetics,immunology Specific Pathogen-Free Organisms Substance P/analysis,biosynthesis Tachykinins/genetics Vaccination
Chemicals
RNA, Messenger Tachykinins Substance P Neurokinin A Ovalbumin Calcitonin Gene-Related Peptide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fischer A
Institute for Anatomy and Cell Biology, Philipps University, Marburg, Germany. [email protected]
McGregor G P
Saria A
Philippin B
Kummer W
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI119039
Email
[email protected]
Published
1996-11-15
Pages
2284-91
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC507678
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI119039 PMC Full Text View journal details
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