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PMID: 8948628 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro.

Nucleic acids research ·Vol. 24 ·No. 22 ·1996-11-15 ·Pages 4387-94

Caldecott KW, Aoufouchi S, Johnson P, Shall S

Abstract

The DNA repair proteins XRCC1 and DNA ligase III are physically associated in human cells and directly interact in vitro and in vivo. Here, we demonstrate that XRCC1 is additionally associated with DNA polymerase-beta in human cells and that these polypeptides also directly interact. We also present data suggesting that poly (ADP-ribose) polymerase can interact with XRCC1. Finally, we demonstrate that DNA ligase III shares with poly (ADP-ribose) polymerase the novel function of a molecular DNA nick-sensor, and that the DNA ligase can inhibit activity of the latter polypeptide in vitro. Taken together, these data suggest that the activity of the four polypeptides described above may be co-ordinated in human cells within a single multiprotein complex.

MeSH Terms
Base Sequence DNA Ligase ATP DNA Ligases/chemistry,genetics,metabolism DNA Polymerase I/chemistry,genetics,metabolism DNA Repair DNA-Binding Proteins/metabolism Electrophoresis, Polyacrylamide Gel Humans Molecular Sequence Data Poly(ADP-ribose) Polymerases/metabolism Poly-ADP-Ribose Binding Proteins X-ray Repair Cross Complementing Protein 1 Xenopus Proteins Zinc Fingers
Chemicals
DNA-Binding Proteins Poly-ADP-Ribose Binding Proteins X-ray Repair Cross Complementing Protein 1 XRCC1 protein, human Xenopus Proteins Poly(ADP-ribose) Polymerases DNA Polymerase I DNA Ligases DNA Ligase ATP DNA ligase III alpha protein, Xenopus LIG3 protein, human
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Caldecott K W
Zeneca Laboratory for Cell and Molecular Biology, School of Biological Sciences, University of Manchester, UK. [email protected]
Aoufouchi S
Johnson P
Shall S
References (24)
24 references, click to expand
  1. Proliferating cell nuclear antigen-dependent abasic site repair in Xenopus laevis oocytes: an alternative pathway of base excision DNA repair.
    Mol Cell Biol. 1994 Sep;14(9):6187-97 PMID: 7915006
  2. Poly(ADP-ribose) polymerase: a molecular nick-sensor.
    Trends Biochem Sci. 1994 Apr;19(4):172-6 PMID: 8016868
  3. Excision of deoxyribose phosphate residues by DNA polymerase beta during DNA repair.
    Science. 1995 Aug 4;269(5224):699-702 PMID: 7624801
  4. Mismatch repair: mechanisms and relationship to cancer susceptibility.
    Trends Biochem Sci. 1995 Oct;20(10):397-401 PMID: 8533151
  5. Nucleotide excision repair and the link with transcription.
    Trends Biochem Sci. 1995 Oct;20(10):402-5 PMID: 8533152
  6. Post-translational modification of poly(ADP-ribose) polymerase induced by DNA strand breaks.
    Trends Biochem Sci. 1995 Oct;20(10):405-11 PMID: 8533153
  7. Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells.
    Nucleic Acids Res. 1995 Dec 11;23(23):4836-43 PMID: 8532526
  8. Requirement of mammalian DNA polymerase-beta in base-excision repair.
    Nature. 1996 Jan 11;379(6561):183-6 PMID: 8538772
  9. Specific interaction of DNA polymerase beta and DNA ligase I in a multiprotein base excision repair complex from bovine testis.
    J Biol Chem. 1996 Jul 5;271(27):16000-7 PMID: 8663274
  10. DNA repair in humans.
    Annu Rev Genet. 1995;29:69-105 PMID: 8825469
  11. Poly(ADP-ribose) synthesis in vitro programmed by damaged DNA. A comparison of DNA molecules containing different types of strand breaks.
    J Biol Chem. 1980 Nov 10;255(21):10502-8 PMID: 6253477
  12. A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange.
    Mutat Res. 1982 Aug;95(2-3):427-40 PMID: 6889677
  13. DNA-mediated transfer of a human DNA repair gene that controls sister chromatid exchange.
    Mol Cell Biol. 1985 Apr;5(4):881-4 PMID: 3990694
  14. Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange.
    Mol Cell Biol. 1990 Dec;10(12):6160-71 PMID: 2247054
  15. Generation of single-nucleotide repair patches following excision of uracil residues from DNA.
    Mol Cell Biol. 1992 Apr;12(4):1605-12 PMID: 1549115
  16. Role of poly(ADP-ribose) formation in DNA repair.
    Nature. 1992 Mar 26;356(6367):356-8 PMID: 1549180
  17. Overproduction and large-scale purification of the human poly(ADP-ribose) polymerase using a baculovirus expression system.
    Gene. 1992 May 15;114(2):279-83 PMID: 1601310
  18. A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges.
    Mutagenesis. 1992 Jul;7(4):265-9 PMID: 1518409
  19. Overproduction of the poly(ADP-ribose) polymerase DNA-binding domain blocks alkylation-induced DNA repair synthesis in mammalian cells.
    EMBO J. 1993 May;12(5):2109-17 PMID: 8491199
  20. Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
    Mol Cell Biol. 1994 Jan;14(1):310-7 PMID: 8264597
  21. An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
    Mol Cell Biol. 1994 Jan;14(1):68-76 PMID: 8264637
  22. Altered DNA ligase III activity in the CHO EM9 mutant.
    Mutat Res. 1994 Mar;314(2):177-86 PMID: 7510367
  23. Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23.
    EMBO J. 1994 Apr 15;13(8):1831-43 PMID: 8168482
  24. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.
    Mol Cell Biol. 1995 Jun;15(6):3206-16 PMID: 7760816
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.22.4387
Email
[email protected]
Published
1996-11-15
Pages
4387-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146288
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/24.22.4387 PMC Full Text View journal details
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