Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato

Science (New York, N.Y.) ·Vol. 274 ·No. 5295 ·1996-12-20 ·Pages 2063-5

Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle DT, Michelmore RW, Staskawicz BJ

Abstract

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two-hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Scofield

S. R. Scofield, J. P. Rathjen, J. H. Chang, D. T. Lavelle, NSF Center for Engineering Plants for Resistance Against Pathogens (CEPRAP), University of California, Davis, CA 95616. C. M. Tobias, Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720. R. W. Michelmore, NSF Center for Engineering Plants for Resistance Against Pathogens and Department of Vegetable Crops, University of California, Davis, CA 95616. B. J. Staskawicz, NSF Center for Engineering Plants for Resistance Against Pathogens and Department of Plant and Microbial Biology, University of California, Davis, CA 94720.

Tobias

Rathjen

Chang

Lavelle

Michelmore

Staskawicz

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.274.5295.2063

Published

1996-12-20

Pages

2063-5

Language

English

Region

United States

NLM ID

0404511

External Links

PubMed source DOI: 10.1126/science.274.5295.2063 View journal details

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Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato

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