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PMID: 8976176 Published · ppublish English Comparative Study Journal Article

Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes.

The Journal of experimental medicine ·Vol. 184 ·No. 6 ·1996-12-01 ·Pages 2207-16

Wang RF, Appella E, Kawakami Y, Kang X, Rosenberg SA

Abstract

The infusion of TIL586 along with interleukin-2 into the autologous patient with metastatic melanoma resulted in the objective regression of tumor. A gene encoding a tumor antigen recognized by TIL586 was previously isolated and shown to encode gp75 or TRP-1. Here we report that TRP-2 was identified as a second tumor antigen recognized by a HLA-A31-restricted CTL clone derived from the TIL586 cell line. The peptide LLPGGRPYR epitope was subsequently identified from the coding region of TRP-2 based on studies of the recognition of truncated TRP-2 cDNAs and the HLA-A31 binding motif. This epitope peptide was capable of sensitizing target cells for lysis by a CTL clone at 1 nM peptide concentration. Although some modified peptides could be recognized by the CTL clone, none were found to be better recognized by T cells than the parental peptide. Like other melamona differentiation antigens, TRP-2 was only expressed in melanoma, melanocytes, and retina, but not in other human tissues tested.

MeSH Terms
Amino Acid Sequence Animals Antigens, Neoplasm/immunology Base Sequence Binding Sites COS Cells Cell Line Epitopes/analysis,immunology Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis HLA-A3 Antigen/immunology Humans Intramolecular Oxidoreductases Isomerases/biosynthesis,chemistry,immunology Lymphocytes, Tumor-Infiltrating/immunology Melanoma/immunology Membrane Glycoproteins Molecular Sequence Data Oxidoreductases Peptide Fragments/chemistry,immunology Protein Biosynthesis Proteins/immunology T-Lymphocytes, Cytotoxic/immunology Transfection
Chemicals
Antigens, Neoplasm Epitopes HLA-A3 Antigen Membrane Glycoproteins Peptide Fragments Proteins Granulocyte-Macrophage Colony-Stimulating Factor Oxidoreductases TYRP1 protein, human tyrosinase-related protein-1 Isomerases Intramolecular Oxidoreductases dopachrome isomerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang R F
National Cancer Institute, Bethesda, Maryland 20892, USA.
Appella E
Kawakami Y
Kang X
Rosenberg S A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.6.2207
Published
1996-12-01
Pages
2207-16
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2211562
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.184.6.2207 PMC Full Text View journal details
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