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PMID: 8978689 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+.

The EMBO journal ·Vol. 15 ·No. 23 ·1996-12-02 ·Pages 6629-40

Ishii K, Kumada K, Toda T, Yanagida M

Abstract

Ubiquitin-dependent proteolysis is required for the onset of anaphase. We show that protein dephosphorylation by protein phosphatase 1 (PP1) is also essential for initiating anaphase in fission yeast. PP1 may directly or indirectly regulate the 20S cyclosome/APC (anaphase-promoting complex) required for anaphase-promoting proteolysis. Using anti-phosphopeptide antibodies, PP1 is shown to be dephosphorylated at the C-terminus, upon the onset of anaphase, for reactivation. sds23+, a novel gene, is a multicopy suppressor for mutations in PP1 and the 20S cyclosome/APC, implying that the gene dosage increase can relieve the requirement for PP1 and the cyclosome/APC for the onset of anaphase. The sds23+ gene is not essential for cell viability, but a mutant with the gene deleted cannot form colonies at 22 and 36 degrees C. In the sds23 deletion mutant, the progression of anaphase and cytokinesis is retarded and cell shape is aberrant. These defects are overcome by plasmids carrying the genes encoding subunits of the 20S cyclosome/APC or PP1. These results demonstrate functions other than promoting anaphase for the components of the 20S cyclosome/APC and also a close functional relationship of Sds23 with PP1 and 20S cyclosome/APC.

MeSH Terms
Amino Acid Sequence Anaphase Base Sequence Cell Cycle Cell Cycle Proteins/chemistry,genetics,metabolism Fungal Proteins/metabolism Gene Deletion Genes, Fungal Mitosis Molecular Sequence Data Mutagenesis, Site-Directed Phosphopeptides/analysis Protein Phosphatase 1 Protein Tyrosine Phosphatases/genetics,metabolism Schizosaccharomyces/cytology,genetics,physiology Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Suppression, Genetic
Chemicals
Cell Cycle Proteins Fungal Proteins Phosphopeptides Schizosaccharomyces pombe Proteins Sds23 protein, S pombe Protein Phosphatase 1 Protein Tyrosine Phosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ishii K
Department of Biophysics, Faculty of Science, Kyoto University, Sakyo-ku, Japan.
Kumada K
Toda T
Yanagida M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-12-02
Pages
6629-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452487
Subset
IM
Databases
GENBANK
D86840
External Links
PubMed source PMC Full Text View journal details
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