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PMID: 8980528 Published · ppublish English Journal Article

Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana.

Plant molecular biology ·Vol. 32 ·No. 4 ·1996-11-00 ·Pages 759-65

Utsugi S, Sakamoto W, Ogura Y, Murata M, Motoyoshi F

Abstract

Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Cloning, Molecular DNA, Complementary/genetics DNA, Plant/genetics Gene Expression Regulation, Plant/physiology Molecular Sequence Data Plant Leaves/chemistry RNA, Messenger/analysis Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
Chemicals
DNA, Complementary DNA, Plant RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Utsugi S
Research Institute for Bioresources, Okayama University, Kurashiki, Japan.
Sakamoto W
Ogura Y
Murata M
Motoyoshi F
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00020217
Published
1996-11-00
Pages
759-65
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
D85190, D85191, D85192, D85193, D85194, X79490
SWISSPROT
P27061
External Links
PubMed source DOI: 10.1007/BF00020217 View journal details
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