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PMID: 8995809 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Corynebacterium coyleae sp. nov., isolated from human clinical specimens.

International journal of systematic bacteriology ·Vol. 47 ·No. 1 ·1997-01-00 ·Pages 92-6

Funke G, Ramos CP, Collins MD

Abstract

Over a 5-year period, six isolates of a previously unknown nonlipophilic coryneform bacterium were isolated from human clinical specimens. The most characteristic phenotypic reactions of these isolates included slow fermentative acid production from glucose but no acid production from maltose and sucrose and a strongly positive CAMP reaction. Chemotaxonomic investigations revealed that meso-diaminopimelic acid and mycolic acids were present, that palmitic, oleic, and stearic acids were the predominant cellular fatty acids, and that the G + C content was 62 to 64 mol%, characteristics which are consistent with assignment to the genus Corynebacterium. Phenotypically, the unknown coryneform bacterium could be readily differentiated from all other Corynebacterium species. A 16S rRNA gene sequence analysis and quantitative DNA-DNA hybridization experiments demonstrated unambiguously that the unknown coryneform bacterium is a member of the genus Corynebacterium and is genotypically distinct from all other members of this genus. Based on phenotypic and genotypic findings, a new species, Corynebacterium coyleae sp.nov., is proposed. The type strain of C coylease is strain DSM 44184 (= CCUG 35014).

MeSH Terms

Adult Aged Bacteriological Techniques Base Composition Corynebacterium/chemistry,genetics,isolation & purification,metabolism Corynebacterium Infections/microbiology Culture Media/metabolism Diaminopimelic Acid/analysis Fatty Acids/analysis Female Fermentation Glucose/metabolism Humans Male Maltose/metabolism Microbial Sensitivity Tests Molecular Sequence Data Mycolic Acids/analysis Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid Sucrose/metabolism

Chemicals

Culture Media Fatty Acids Mycolic Acids RNA, Ribosomal, 16S Sucrose Diaminopimelic Acid Maltose Glucose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Funke G

Department of Medical Microbiology, University of Zürich, Switzerland. [email protected]

Ramos C P

Collins M D

Article Info

Journal

International journal of systematic bacteriology

Abbr.

Int J Syst Bacteriol

ISSN

0020-7713

DOI

10.1099/00207713-47-1-92

[email protected]

Published

1997-01-00

Pages

92-6

Language

English

Region

England

NLM ID

0042143

Subset

Databases

GENBANK

X80499, X80500, X81871, X81874, X82055, X82493, X83955, X84240, X84241, X84244, X84245, X84246, X84248, X84249, X84250, X84251, X84252, X84253, X84255, X84257, X84258, X84438, X84440, X84441, X84443, X84444, X84678, X86688, X96497

External Links

PubMed source DOI: 10.1099/00207713-47-1-92

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Corynebacterium coyleae sp. nov., isolated from human clinical specimens.

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