Characterization of human platelet receptors for atrial natriuretic peptide: evidence for clearance receptors.

Characterization of human platelet receptors for atrial natriuretic peptide (ANP) has been performed using two different methods. First, intracellular cGMP levels were measured after platelet stimulation with humanANP (hANP) and CNP, in order to detect the presence of ANP-A or ANP-B receptors. The intracellular cGMP content was not modified either after hANP or CNP platelet stimulation and thus indicated the lack of biological active receptors on these cells. Second, displacements of 125I-hANP were similar when induced by hANP or C4-23ANP (specific ligand of clearance receptor). Consequently, these results confirmed that human platelet membranes bore only clearance receptors for atrial natriuretic peptide.