Home LiteratureArticle Details
PMID: 900917 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro uptake of gentamicin by rat renal cortical tissue.

Antimicrobial agents and chemotherapy ·Vol. 12 ·No. 2 ·1977-08-00 ·Pages 192-4

Hsu CH, Kurtz TW, Weller JM

Abstract

The mechanism of gentamicin uptake in vitro by renal cortical slices of rat kidney was investigated. The cortical-slice-uptake ratio of gentamicin concentration in 1.0 g of tissue water to that of 1.0 ml of incubation medium (SW/M) was 1.44 +/- 0.04. The uptake of gentamicin was inhibited by 2 x 10(-5) M dinitrophenol (SW/M = 1.03 +/- 0.04) and by anoxia (SW/M = 1.01 +/- 0.04). The results indicate that aerobic phosphorylation is required to transport gentamicin into the cells. The uptake of p-aminohippurate and tetraethylammonium chloride by renal cortical slices was not affected by gentamicin.

MeSH Terms
Animals Gentamicins/metabolism,pharmacology In Vitro Techniques Kidney Cortex/drug effects,metabolism Rats Tetraethylammonium Compounds/metabolism Water/metabolism p-Aminohippuric Acid/metabolism
Chemicals
Gentamicins Tetraethylammonium Compounds Water p-Aminohippuric Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hsu C H
Kurtz T W
Weller J M
References (11)
11 references, click to expand
  1. Renal tubular transport: accumulation of p-aminohippurate by rabbit kidney slices.
    Am J Physiol. 1950 Apr 1;161(1):181-90 PMID: 15419315
  2. RENAL TUBULAR MECHANISMS FOR EXCRETION OF ORGANIC ACIDS AND BASES.
    Am J Med. 1964 May;36:743-62 PMID: 14141450
  3. Persistence of sisomicin and gentamicin in renal cortex and medulla compared with other organs and serum of rats.
    Kidney Int. 1976 Dec;10(6):444-9 PMID: 1011538
  4. Editorial: Intrarenal antibiotic distribution in health and disease.
    Kidney Int. 1974 Sep;6(3):131-7 PMID: 4608832
  5. Renal parenchymal accumulation of aminoglycoside antibiotics in rats.
    J Infect Dis. 1974 Dec;130(6):656-9 PMID: 4427081
  6. Potentiation of gentamicin nephrotoxicity by metabolic acidosis.
    Proc Soc Exp Biol Med. 1974 Jul;146(3):894-7 PMID: 4841206
  7. Relationship between cephaloridine and p-aminohippurate transport in the kidney.
    Am J Physiol. 1973 Nov;225(5):1114-7 PMID: 4745209
  8. Effect of organic acid transport inhibitors on renal cortical uptake and proximal tubular toxicity of cephaloridine.
    J Pharmacol Exp Ther. 1972 May;181(2):250-6 PMID: 5030671
  9. The role of tubular necrosis in the pathophysiology of acute renal failure.
    Nephron. 1976;17(3):204-14 PMID: 940626
  10. Pharmacokinetics of gentamicin: distribution and plasma and renal clearance.
    J Infect Dis. 1971 Dec;124 Suppl:S70-6 PMID: 5126251
  11. The toxic effect of ammonia on renal cortical tubule function in vitro.
    J Lab Clin Med. 1968 Apr;71(4):561-72 PMID: 5651361
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.12.2.192
Published
1977-08-00
Pages
192-4
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC429883
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.12.2.192 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2